Anticorps Monoclonal anti-NeutraControl TGF beta 1

NeutraControl TGF beta 1 Monoclonal Antibody for Non-Neutralization, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

Non-Neutralization, ELISA

Conjugaison

Non conjugué

CloneNo.

1D3D11

N° de cat : 69512-1-Ig

Synonymes

CED, DPD1, LAP, TGF ?1, TGF beta 1, TGFB, TGFB1, TGFbeta

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Galerie de données de validation

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  Non-Neutralization experiment of HT-2 cells using 69512-1-Ig

  Recombinant human TGF beta 1 (Cat.NO. HZ-1011) inhibits IL-4 induced proliferation of mouse HT-2 cells in a dose dependent nammer (blue curve, refer to bottom X-left Y). The activity of human TGF beta 1 (0.5 ng/mL HZ-1011) is neutralized by mouse anti-human TGF beta 1 antibody 69012-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 1-5 μg/mL.The NeutraControl mouse anti-human TGF beta 1 monoclonal antibody 69512-1-Ig could recognize human TGF beta 1, but could not neutralize human TGF beta 1 in the range of 0.0061-100 μg/mL(green curve, refer to top X-right Y).

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  ELISA experiment of Recombinant protein using 69512-1-Ig

  Indirect ELISA was carried out by coating recombinant Human TGF beta 1 (Cat.NO. HZ-1011) at 70 ng/well followed by blocking and adding serial diluted TGF beta 1 antibody 69012-1-Ig and 69512-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

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Informations sur le produit

69512-1-Ig cible TGF beta 1 dans les applications de Non-Neutralization, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: human Humankine TGF beta 1 protein HZ-1011
• Nom complet: transforming growth factor, beta 1
• Symbole du gène: TGFB1
• Identification du gène (NCBI): 7040
• Conjugaison: Non conjugué
• Forme: Lyophilized Powder
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS stérile.
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Informations générales

TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1).

This antibody is a neutralizing control antibody for TGF beta 1, the immunogen is the same as Neutrakine 69012-1-Ig but could not neutralize human TGF beta 1.

Produits associés

NeutraKine® TGF beta 1 Monoclonal antibody

69012-1-Ig