Anticorps Polyclonal de lapin anti-TGF beta 1

TGF beta 1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 26155-1-AP

Synonymes

TGF beta1, Tgfb, Tgfb 1, Tgfb1, TGF-beta 1, TGFbeta1, TGF-beta1, TGF-β, TGFβ1, TGF-β1

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Galerie de données de validation

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  WB analysis using 26155-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26155-1-AP (Tgfb1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 26155-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26155-1-AP (Tgfb1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26155-1-AP

  NIH3T3 cells were subjected to SDS PAGE followed by western blot with 26155-1-AP (Tgfb1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules L-929, cellules NIH/3T3, cellules RAW 264.7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

26155-1-AP cible TGF beta 1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TGF beta 1 Protéine recombinante Ag24078
• Nom complet: transforming growth factor, beta 1
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: NM-011577
• Symbole du gène: Tgfb1
• Identification du gène (NCBI): 21803
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TGF beta 1 is a potent cytokine capable of regulating multiple cellular processes including cell proliferation, differentiation, wound healing, and immune response. TGF beta 1 is identified in various immune cells and its activity varies in different cell types or cells at different developmental stages1. TGF beta 1 functions by binding to the TGFβ1 receptors, which subsequently activate the canonical SMAD pathway or non-canonical signaling cascades, such as the mitogen-activated protein kinase (MAPK) pathway, phosphatidylinositol-3 kinase and AKT (PI3K-AKT), and Rho family of GTPases (Rho GTPase). What is special about TGF beta 1 is its context-dependent nature that is particularly true for tumors. Full-length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB (12, 25, 45 to 65 kDa).