Anticorps Polyclonal de lapin anti-Thyroglobulin

Thyroglobulin Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 21714-1-AP

Synonymes

AITD3, TG, TGN, thyroglobulin

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Galerie de données de validation

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  IHC staining of human thyroid using 21714-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 21714-1-AP (Thyroglobulin antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human thyroid using 21714-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 21714-1-AP (Thyroglobulin antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human thyroid cancer using 21714-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21714-1-AP (Thyroglobulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 21714-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21714-1-AP (Thyroglobulin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IHC: tissu thyroïdien humain, tissu de cancer de la thyroïde humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

21714-1-AP cible Thyroglobulin dans les applications de WB, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Thyroglobulin Protéine recombinante Ag16343
• Nom complet: thyroglobulin
• Masse moléculaire calculée: 2768 aa, 305 kDa
• Numéro d’acquisition GenBank: BC140933
• Symbole du gène: Thyroglobulin
• Identification du gène (NCBI): 7038
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis.

Publications

Species	Application	Title
human	WB	Mol Cell Proteomics; Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis; Authors - Madison T. Wright
human	WB	Oncotarget; HER inhibitor promotes BRAF/MEK inhibitor-induced redifferentiation in papillary thyroid cancer harboring BRAFV600E.; Authors - Lingxiao Cheng
	CoIP	Mol Cell Proteomics; Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis.; Authors - Madison T Wright
human	IF	J Lipid Res; Differential contributions of choline phosphotransferases CPT1 and CEPT1 to the biosynthesis of choline phospholipid.; Authors - Yasuhiro Horibata
human	WB	Cancer Lett; Epigenetic inhibition of CTCF by HN1 promotes dedifferentiation and stemness of anaplastic thyroid cancer; Authors - Zongfu Pan