Anticorps Polyclonal de lapin anti-TFPI

TFPI Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 20865-1-AP

Synonymes

TFPI1, TFI, Lipoprotein-associated coagulation inhibitor, LACI, Extrinsic pathway inhibitor

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Galerie de données de validation

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  WB analysis of HeLa using 20865-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20865-1-AP (TFPI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20865-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20865-1-AP (TFPI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 20865-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TFPI antibody (20865-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

20865-1-AP cible TFPI dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TFPI Protéine recombinante Ag14913
• Nom complet: tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
• Masse moléculaire calculée: 251aa,29 kDa; 304aa,35 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC015514
• Symbole du gène: TFPI
• Identification du gène (NCBI): 7035
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tissue factor pathway inhibitor (TFPI) is a critical anticoagulant protein present in endothelium and platelets. TFPI is produced as two major isoforms in humans, TFPIa and TFPIb that result from alternative splicing. The TFPIb isoform localizes to the endothelium surface where it is a potent inhibitor of tissue factor-factor VIIa complexes that initiate blood coagulation. The TFPIa isoform is present in platelets. TFPI is a Kunitz-type serine protease inhibitor that exerts anticoagulant activity by blocking early procoagulant stimulia. TFPI can enhance anti-thrombotic treatment in sepsis, inflammatory diseases, and cardiovascular diseases.

Publications

Species	Application	Title
human	WB	Gene; ADTRP regulates TFPI expression via transcription factor POU1F1 involved in coronary artery disease.; Authors - Chunyan Luo
human	IHC	Clin Appl Thromb Hemost; Endocardial Changes in Nonvalvular Atrial Fibrillation Without Atrial Thrombus-Thrombomodulin and Tissue Factor Pathway Inhibitor.; Authors - Kang An
human	WB	Thromb Res; Activated protein C, protein S, and tissue factor pathway inhibitor cooperate to inhibit thrombin activation; Authors - Xian Li