Anticorps Polyclonal de lapin anti-TFIP11

TFIP11 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 14436-1-AP

Synonymes

bK445C9.6, STIP, STIP 1, TFIP11, TIP39

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Galerie de données de validation

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  WB analysis of HeLa using 14436-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14436-1-AP (TFIP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14436-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14436-1-AP (TFIP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14436-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14436-1-AP (TFIP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14436-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14436-1-AP (TFIP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 14436-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14436-1-AP (TFIP11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules K-562, cellules MCF-7, tissu testiculaire de souris
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

14436-1-AP cible TFIP11 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TFIP11 Protéine recombinante Ag5821
• Nom complet: tuftelin interacting protein 11
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 97 kDa
• Numéro d’acquisition GenBank: BC033080
• Symbole du gène: TFIP11
• Identification du gène (NCBI): 24144
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TFIP11, also named as Tuftelin-interacting protein 11 or Septin and tuftelin-interacting protein 1, is a 837 amino acid protein, which contains one G-patch domain and belongs to the TFP11/STIP family. TFIP11 may localizes in the nucleus and cytoplasm. TFIP11 is involved in pre-mRNA splicing, specifically in spliceosome disassembly during late-stage splicing events. TFIP11 may play a role in the differentiation of ameloblasts and odontoblasts or in the forming of the enamel extracellular matrix.