Anticorps Polyclonal de lapin anti-Trefoil factor 3

• Phare
• Validé par KD/KO

Trefoil factor 3 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 23277-1-AP

Synonymes

HITF, hP1.B, Intestinal trefoil factor, ITF, Polypeptide P1.B, TFF3, TFI, Trefoil factor 3, trefoil factor 3 (intestinal)

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Galerie de données de validation

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  IHC staining of human colon using 23277-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23277-1-AP (Trefoil factor 3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 23277-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23277-1-AP (Trefoil factor 3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 23277-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 23277-1-AP (Trefoil factor 3 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 23277-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 23277-1-AP (Trefoil factor 3 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 23277-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 23277-1-AP (Trefoil factor 3 antibody at dilution of 1:50.

• 

  IHC staining of human colon using 23277-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 23277-1-AP (Trefoil factor 3 antibody at dilution of 1:50.

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Applications testées

• Résultats positifs en IHC: tissu de côlon humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Informations sur le produit

23277-1-AP cible Trefoil factor 3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Trefoil factor 3 Protéine recombinante Ag9543
• Nom complet: trefoil factor 3 (intestinal)
• Masse moléculaire calculée: 80 aa, 9 kDa
• Numéro d’acquisition GenBank: BC017859
• Symbole du gène: TFF3
• Identification du gène (NCBI): 7033
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Trefoil factor 3 (TFF3) belongs to the TFF-domain peptide family which consists of three small secreted proteins (TFF1, TFF2, TFF3) that are expressed by mucous-secreting epithelia. TFF3 is a major constituent in the goblet cells in small and large intestines, especially a typical secretary peptide of the normal human antral and pyloric gastric mucosa. TFF3 is essential in regulating cell migration and maintaining normal GI mucosal integrity. TFF3 has been reported to be overexpressed at the gene and the protein level in human neoplasms such as prostate, breast, and colon cancer. TFF3 is involved in tumor cell scattering, angiogenesis, and invasion.

Publications

Species	Application	Title
human	WB	EMBO J; The chromatin remodeler SRCAP promotes self-renewal of intestinal stem cells.; Authors - Buqing Ye
	WB,IHC	Neoplasia; Downregulation of Notch Signaling in Kras-Induced Gastric Metaplasia.; Authors - Wen-Cheng Chung
human	IHC	Rhinology; Expression of Trefoil factor family peptides in the nasal allergic mucosa.; Authors - Miyahara Nobuyuki N
human	WB	J Histotechnol; Trefoil factor 3 knock-down prevents autophagy-related gene 12 elevation in colon adenocarcinoma.; Authors - Zhuo Zhang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iru (Verified Customer) (06-21-2019)

There blot doesn't come out clean.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fallopian tube epithelial cells

Trefoil factor 3 Antibody Western Blot, validation (1:1000 dilution) in Fallopian tube epithelial cells (Cat no:23277-1-AP)