Anticorps Polyclonal de lapin anti-TFAM

• Phare
• Validé par KD/KO

TFAM Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF/ICC, IP, chIP, ELISA

Conjugaison

Non conjugué

Publications

126

Avis (2)

N° de cat : 22586-1-AP

Synonymes

Cell and organelle markers, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker, MtTF1, mtTFA, TCF 6, TCF6, TCF6L1, TCF6L2, TCF6L3, TFAM, Transcription factor 6, Transcription factor 6 like 2

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Galerie de données de validation

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  WB analysis using 22586-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22586-1-AP (TFAM antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis using 22586-1-AP

  ATDC-5 cells were subjected to SDS PAGE followed by western blot with 22586-1-AP (TFAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 22586-1-AP

  IP result of anti-TFAM (IP:22586-1-AP, 4ug; Detection:22586-1-AP 1:800) with HEK-293 cells lysate 3200ug.

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  IHC staining of human colon cancer using 22586-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22586-1-AP (TFAM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 22586-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22586-1-AP (TFAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 22586-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFAM antibody (22586-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 22586-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFAM antibody (22586-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules ATDC-5, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules MCF-7
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 115 publications below
• IHC: See 10 publications below
• IF: See 9 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

22586-1-AP cible TFAM dans les applications de WB, IHC, IF/ICC, IP, chIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, poisson-zèbre, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TFAM Protéine recombinante Ag18299
• Nom complet: transcription factor A, mitochondrial
• Masse moléculaire calculée: 246 aa, 29 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC126366
• Symbole du gène: TFAM
• Identification du gène (NCBI): 7019
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.

Publications

Species	Application	Title
	WB	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
human	WB	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
	IHC	Adv Mater; Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine; Authors - Peng Lou
	WB，IHC	Circulation; Extracellular Tuning of Mitochondrial Respiration Leads to Aortic Aneurysm.; Authors - Jorge Oller
	IHC	Cell Metab; Mitochondrial Damage and Activation of the STING Pathway Lead to Renal Inflammation and Fibrosis.; Authors - Ki Wung Chung
human	WB	Nat Cell Biol; Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.; Authors - Timo Rey

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sebastian (Verified Customer) (10-25-2023)

Good detection signals for TFAM in western blot of liver from old and young mice, some unspecific bands were slightly visible

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: liver

FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain