Anticorps Polyclonal de lapin anti-TEX14

TEX14 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 18351-1-AP

Synonymes

SGK307, SGK307, Sugen kinase 307, testis expressed 14, Testis expressed protein 14, Testis expressed sequence 14, TEX14



Applications testées

Résultats positifs en IHCtissu testiculaire de souris,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Immunohistochimie (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

18351-1-AP cible TEX14 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène TEX14 Protéine recombinante Ag13177
Nom complet testis expressed 14
Masse moléculaire calculée957aa,107 kDa; 1497aa,168 kDa
Poids moléculaire observé 180-200 kDa, 106 kDa
Numéro d’acquisition GenBankBC040526
Symbole du gène TEX14
Identification du gène (NCBI) 56155
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tex14 is required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Tex14 acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges.Tex14 also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment. Tex14 has several variant isoforms with the MW from about 100 kDa to 168 kDa.

Protocole

Product Specific Protocols
WB protocol for TEX14 antibody 18351-1-APDownload protocol
IHC protocol for TEX14 antibody 18351-1-APDownload protocol
IP protocol for TEX14 antibody 18351-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Int J Mol Sci

MCF7 Spheroid Development: New Insight about Spatio/Temporal Arrangements of TNTs, Amyloid Fibrils, Cell Connections, and Cellular Bridges.

Authors - Laura Pulze
human,mouseIF

FASEB J

Dazl determines primordial follicle formation through the translational regulation of Tex14.

Authors - Roseanne Rosario
mouseIHC

Methods Mol Biol

Whole-mount immunohistochemistry to study spermatogonial stem cells and spermatogenic lineage development in mice, monkeys, and humans.

Authors - Kathrin Gassei
mouseIF

Cell Death Dis

Specific deletion of protein phosphatase 6 catalytic subunit in Sertoli cells leads to disruption of spermatogenesis.

Authors - Wen-Long Lei
mouseIF

Cells

The Male Mouse Meiotic Cilium Emanates from the Mother Centriole at Zygotene Prior to Centrosome Duplication

Authors - Pablo López-Jiménez