Anticorps Polyclonal de lapin anti-TERF1

TERF1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 11899-1-AP

Synonymes

hTRF1 AS, NIMA interacting protein 2, PIN2, t TRF1, Telomeric protein Pin2/TRF1, TERF1, TRBF1, TRF, TRF1, TTAGGG repeat binding factor 1

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Galerie de données de validation

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  WB analysis of HEK-293 using 11899-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11899-1-AP (TERF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11899-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11899-1-AP (TERF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11899-1-AP

  IP result of anti-TERF1 (IP:11899-1-AP, 4ug; Detection:11899-1-AP 1:300) with HEK-293 cells lysate 2000ug.

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  IHC staining of human gliomas using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11899-1-AP (TERF1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11899-1-AP (TERF1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de gliome humain, tissu cardiaque humain, tissu cérébral humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

11899-1-AP cible TERF1 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TERF1 Protéine recombinante Ag2491
• Nom complet: telomeric repeat binding factor (NIMA-interacting) 1
• Masse moléculaire calculée: 439 aa, 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC029378
• Symbole du gène: TERF1
• Identification du gène (NCBI): 7013
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Telomeres are nucleoprotein complexes located at chromosome ends that consist of tandem arrays of TTAGGG repeats bound to specific proteins. Telomeres protect chromosome ends from degradation and end-to-end fusions, prevent activation of DNA damage checkpoints, and modulate the maintenance of telomeric DNA by telomerase. TERF1 (telomeric repeat-binding factor 1) binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. It's one component of the shelterin complex, which involved in the regulation of telomere length and protection.

Publications

Species	Application	Title
mouse	IF	PLoS Genet; IGSF11 is required for pericentric heterochromatin dissociation during meiotic diplotene.; Authors - Bo Chen
human	WB	CNS Neurosci Ther; FHOD1 is upregulated in glioma cells and attenuates ferroptosis of glioma cells by targeting HSPB1 signaling; Authors - Fan Zhang
human	WB	Sci Adv; Structural basis of telomeric nucleosome recognition by shelterin factor TRF1; Authors - Hongmiao Hu
rat	IHC	Eur J Pharmacol; Pentoxifylline protects against cerebral ischaemia-reperfusion injury through ferroptosis regulation via the Nrf2/SLC7A11/GPX4 signalling pathway; Authors - Pei Li