Anticorps Polyclonal de lapin anti-TEAD4

• Phare
• Validé par KD/KO

TEAD4 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

22

Avis (1)

N° de cat : 12418-1-AP

Synonymes

EFTR 2, hRTEF 1B, RTEF1, TCF13L1, TEA domain family member 4, TEAD 4, TEAD4, TEF 3, TEF3, TEFR 1, Transcription factor 13 like 1, Transcription factor RTEF 1

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Galerie de données de validation

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  WB analysis using 12418-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12418-1-AP (TEAD4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules BGC-823, cellules HepG2, cellules MCF-7, cellules MDA-MB-231

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 19 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

12418-1-AP cible TEAD4 dans les applications de WB, IP, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TEAD4 Protéine recombinante Ag3059
• Nom complet: TEA domain family member 4
• Masse moléculaire calculée: 434 aa, 48 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC015497
• Symbole du gène: TEAD4
• Identification du gène (NCBI): 7004
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TEAD4 belongs to a protein family that share the TEA/ATTS domain. The transcriptional activity of TEAD4 required several regions of the proteins , a TBP-associated factor and a limiting transcriptional intermediary facotrs. TEAD4 also preforms an essential role in the Hippo signaling pathway. TEAD4 regulates gene expression of YAP1 and WWTR1/TAZ, thus mediating cell proliferation, migration and epithelial mesenchymal transition induction. The calculated molecular weight of TEAD4 is 48 kDa, but molecular weight of TEAD4 is detected about 50-55 kDa.

Publications

Species	Application	Title
mouse	WB	J Cell Biol; Thyroid hormone regulates muscle fiber type conversion via miR-133a1.; Authors - Duo Zhang
human	WB	Cell Mol Biol Lett; KIAA1429-mediated m6A modification of CHST11 promotes progression of diffuse large B-cell lymphoma by regulating Hippo-YAP pathway; Authors - Xiaomin Chen
human	WB	Br J Pharmacol; An agonist of the adenosine A2A receptor, CGS21680, promotes corneal epithelial wound healing via the YAP signalling pathway; Authors - Qiuqin Sun
human,mouse	WB,IF	Clin Sci (Lond); IGFBP7 promotes endothelial cell repair in the recovery phase of acute lung injury; Authors - Rui He
human	CoIP	J Cell Mol Med; PMID: 31559693; Authors - Yangyang Yao
human	WB	Carcinogenesis; A yes-associated protein 1-Notch1 positive feedback loop promotes breast cancer lung metastasis by attenuating the Bone morphogenetic protein 4-SMAD family member 1/5 signaling; Authors - Lin Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Pramod Bahadur (Verified Customer) (03-05-2024)

We used this antibody for PC3 cell lines with vector control, PDEF over expression and PDEF TWIST overexpression

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: PC3

TEAD4 Antibody Western Blot validation (1:2500 dilution) in PC3 (Cat no:12418-1-AP)