Anticorps Polyclonal de lapin anti-TDO2

• Phare
• Validé par KD/KO

TDO2 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

34

Avis (2)

N° de cat : 15880-1-AP

Synonymes

TDO, TDO2, TO, TRPO, Tryptamin 2,3 dioxygenase, tryptophan 2,3 dioxygenase, Tryptophan oxygenase, Tryptophan pyrrolase, Tryptophanase

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Galerie de données de validation

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  WB analysis using 15880-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15880-1-AP (TDO2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15880-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15880-1-AP (TDO2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 15880-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15880-1-AP (TDO2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 15880-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15880-1-AP (TDO2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15880-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15880-1-AP (TDO2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 15880-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using TDO2 antibody (15880-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu hépatique de rat
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 30 publications below
• IHC: See 10 publications below
• IF: See 5 publications below
• FC: See 1 publications below

Informations sur le produit

15880-1-AP cible TDO2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TDO2 Protéine recombinante Ag8642
• Nom complet: tryptophan 2,3-dioxygenase
• Masse moléculaire calculée: 406 aa, 48 kDa
• Poids moléculaire observé: 40-50 kDa
• Numéro d’acquisition GenBank: BC005355
• Symbole du gène: TDO2
• Identification du gène (NCBI): 6999
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TDO2 (tryptophan 2,3-dioxygenase), IDO1 (indoleamine 2,3-dioxygenase 1) and IDO2 are three enzymes that catalyze the amino acid tryptophan into kynurenine in the kynurenine pathway (PMID: 23090118, 28469468). TDO2 plays an important role in neurological diseases such as Alzheimer's disease, Parkinson's disease and autism. Overexpression of TDO2 promoted tumor cell survival and was correlated with tumor grade and poor prognosis in triple negative breast cancer, brain tumors and esophageal squamous cell carcinoma. TDO2 may be a potential therapeutic target in some cancers. (PMID: 21976023, 30134247)

Publications

Species	Application	Title
human, mouse	WB	Cancer Cell; Inhibition of De Novo NAD(+) Synthesis by Oncogenic URI Causes Liver Tumorigenesis through DNA Damage.; Authors - Krishna S Tummala ; KD Validated
human	WB	Nat Commun; Engineered MED12 mutations drive leiomyoma-like transcriptional and metabolic programs by altering the 3D genome compartmentalization; Authors - Kadir Buyukcelebi
human,rat	WB,IHC,IF	Br J Pharmacol; Tryptophan 2,3-dioxygenase 2 plays a key role in regulating the activation of fibroblast-like synoviocytes in autoimmune arthritis.; Authors - Yan Chang ; KD Validated
mouse	WB	Front Immunol; DSS-induced colitis activates the kynurenine pathway in serum and brain by affecting IDO-1 and gut microbiota; Authors - Li-Ping Zhao
human	WB,IHC	Fertil Steril; Tryptophan catabolism is dysregulated in leiomyomas.; Authors - Tsai-Der Chuang
mouse	WB	J Med Chem; Discovery of 1-(Hetero)aryl-β-carboline Derivatives as IDO1/TDO Dual Inhibitors with Antidepressant Activity; Authors - Yu Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hala (Verified Customer) (03-10-2021)

works veryyy gooddd

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000 - 1/2000
• Cell Tissue Type: hepatoblastoma cells transduced with lentivirus expressing TDO2

FH

Hala (Verified Customer) (12-15-2020)

so efficient

• Applications: Western Blot
• Primary Antibody Dilution: 1/750 - 1/1000
• Cell Tissue Type: liver cells transduced with lentivirus expressing TDO2

TDO2 Antibody Western Blot validation (1/750 - 1/1000 dilution) in liver cells transduced with lentivirus expressing TDO2 (Cat no:15880-1-AP)