Anticorps Polyclonal de lapin anti-TCF4/TCF7L2

TCF4/TCF7L2 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

23

N° de cat : 13838-1-AP

Synonymes

HMG box transcription factor 4, hTCF 4, T cell factor 4, TCF 4, TCF4, TCF4/TCF7L2, TCF7L2, Transcription factor 7 like 2

---

Galerie de données de validation

• 

  WB analysis using 13838-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF4/TCF7L2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13838-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 13838-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 13838-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 13838-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13838-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13838-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13838-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 13838-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 13838-1-AP

  IP result of anti-TCF4/TCF7L2 (IP:13838-1-AP, 4ug; Detection:13838-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

---

Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HEK-293, cellules HepG2, tissu cardiaque de souris, tissu cardiaque humain, tissu cérébral humain, tissu hépatique de rat, tissu hépatique humain, tissu placentaire de souris, tissu rénal de souris
• Résultats positifs en IP: tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 21 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 2 publications below

Informations sur le produit

13838-1-AP cible TCF4/TCF7L2 dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TCF4/TCF7L2 Protéine recombinante Ag4786
• Nom complet: transcription factor 7-like 2 (T-cell specific, HMG-box)
• Masse moléculaire calculée: 619 aa, 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC032656
• Symbole du gène: TCF4
• Identification du gène (NCBI): 6934
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Cell Mol Immunol; TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal; Authors - Jing Wang
human	WB	Oncogene; The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer.; Authors - Kai Li
human	WB	Sci Rep; TCF7L2 involvement in estradiol- and progesterone-modulated islet and hepatic glucose homeostasis.; Authors - Fengqin Dong
human	WB,IF,CoIP	Front Cell Dev Biol; Twist1 Contributes to the Maintenance of Some Biological Properties of Dermal Papilla Cells in vitro by Forming a Complex With Tcf4 and β-Catenin.; Authors - Nanlan Yu
human	WB	Mol Cancer Res; Wnt/β-Catenin Signaling Axis Is Required for TFEB-Mediated Gastric Cancer Metastasis and Epithelial-Mesenchymal Transition.; Authors - Shuxuan Li
human	WB	J Biol Chem; HBP1-mediated regulation of p21 through Mdm2/p53 and TCF4/EZH2 pathways and its impact on cell senescence and tumorigenesis.; Authors - Yifan Chen