Anticorps Polyclonal de lapin anti-TBX4/5

TBX4/5 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

11

Avis (1)

N° de cat : 13178-1-AP

Synonymes

HOS, T box 5, T box protein 5, TBX5

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Galerie de données de validation

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  WB analysis of HeLa using 13178-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13178-1-AP (TBX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13178-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13178-1-AP (TBX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13178-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13178-1-AP (TBX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 13178-1-AP

  Immunofluorescent analysis of HepG2 cells using 13178-1-AP (TBX5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules MCF-7
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IF: See 3 publications below

Informations sur le produit

13178-1-AP cible TBX4/5 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TBX4/5 Protéine recombinante Ag3845
• Nom complet: T-box 5
• Masse moléculaire calculée: 518 aa, 57 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC027942
• Symbole du gène: TBX5
• Identification du gène (NCBI): 6910
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

T-box genes encode a family of transcription factors that have been identified in all metazoans and which play diverse roles during embryonic development. TBX5, a member of T-box family, has an essential role in the initiation of limb outgrowth and heart development. Genetic variation near TBX5 that associates with PR and/or QRS interval variation, which suggests that TBX5 plays a role in CCS function in the general population. Because 77 % amino acid sequence of TBX5 is identical to TBX4, this antibody recognizes both TBX4 and TBX5.

Publications

Species	Application	Title
human	IF	Eur Respir J; Single-cell RNA sequencing reveals that BMPR2 mutation regulates right ventricular function via ID genes.; Authors - Mingxia Du
human	WB	Mol Biol Evol; Functional and Adaptive Significance of Promoter Mutations that Affect Divergent Myocardial Expressions of TRIM72 in Primates.; Authors - Yuanqing Feng
mouse	WB	Environ Sci Technol; Perfluorinated Iodine Alkanes Promoted Neural Differentiation of mESCs by Targeting miRNA-34a-5p in Notch-Hes Signaling.; Authors - Zhihua Ren
		Stem Cell Reports; Soft Matrix Promotes Cardiac Reprogramming via Inhibition of YAP/TAZ and Suppression of Fibroblast Signatures.; Authors - Shota Kurotsu
human	IF	iScience; A dual SHOX2:GFP; MYH6:mCherry knockin hESC reporter line for derivation of human SAN-like cells.; Authors - Zaniar Ghazizadeh
mouse	WB	Sci Rep; Stoichiometric optimization of Gata4, Hand2, Mef2c, and Tbx5 expression for contractile cardiomyocyte reprogramming.; Authors - Zhentao Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Young-Jae (Verified Customer) (12-06-2018)

Working good for western blot

• Applications: Western Blot,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: fibroblasts overexpressed with Tbx5