Anticorps Polyclonal de lapin anti-TBX21,T-bet

TBX21,T-bet Polyclonal Antibody for WB, IP, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IF, IHC, ELISA, FC (Intra)

Conjugaison

Non conjugué

Publications

23

N° de cat : 13700-1-AP

Synonymes

T bet, T box 21, T box protein 21, T PET, TBET, TBLYM, TBX21, TBX21,T bet, TBX21,T-bet, Transcription factor TBLYM

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Galerie de données de validation

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  WB analysis of A549 using 13700-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13700-1-AP (TBX21,T-bet antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 13700-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 13700-1-AP (TBX21,T-bet antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 13700-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13700-1-AP (TBX21,T-bet Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse thymus using 13700-1-AP

  IP result of anti-TBX21,T-bet (IP:13700-1-AP, 4ug; Detection:13700-1-AP 1:300) with mouse thymus tissue lysate 2800ug.

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  IHC staining of human tonsillitis using 13700-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13700-1-AP (TBX21,T-bet antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 13700-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13700-1-AP (TBX21,T-bet antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of NK92 using 13700-1-AP

  1X10^6 NK92 were intracellularly stained with 0.4 ug Anti-Human TBX21,T-bet (13700-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules RAW 264.7, tissu de thymus de souris
• Résultats positifs en IP: tissu de thymus de souris
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): NK92
• Résultats positifs en cytométrie: NK92

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 17 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

13700-1-AP cible TBX21,T-bet dans les applications de WB, IP, IF, IHC, ELISA, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TBX21,T-bet Protéine recombinante Ag4618
• Nom complet: T-box 21
• Masse moléculaire calculée: 535 aa, 58 kDa
• Poids moléculaire observé: 60-62 kDa
• Numéro d’acquisition GenBank: BC039739
• Symbole du gène: TBX21/T-bet
• Identification du gène (NCBI): 30009
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TBX21 is a transcription factor that drives the Th1 immune response primarily through promoting expression of the IFN-γ gene. TBX21 initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. It has also been shown to play an influential role in inflammatory processes such as inflammatory bowel disease, autoimmune diseases such as rheumatoid arthritis, and immune-mediated conditions like asthma.

Publications

Species	Application	Title
human	WB	Cell; Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria.; Authors - Rui Yang ; KD Validated
rat	WB	Int Immunopharmacol; Modulating Th1/Th2 drift in asthma-related immune inflammation by enhancing bone mesenchymal stem cell homing through targeted inhibition of the Notch1/Jagged1 signaling pathway; Authors - Wang Kun
human	WB	Int J Cardiol; Endothelial microparticles exert differential effects on functions of Th1 in patients with acute coronary syndrome.; Authors - Lu Yongguang Y
mouse	WB	Mol Nutr Food Res; A High MCT-Based Ketogenic Diet Suppresses Th1 and Th17 Responses to Ameliorate Experimental Autoimmune Encephalomyelitis in Mice by Inhibiting GSDMD and JAK2-STAT3/4 Pathways; Authors - Qianye Zhang
rat	WB	Biomed Pharmacother; Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity.; Authors - Yu Xia
human	WB	Front Pharmacol; Targeting Wnt Signaling in the Tumor Immune Microenvironment to Enhancing EpCAM CAR T-Cell therapy.; Authors - Weizhen Li