• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-TBC1D5

TBC1D5 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 17078-1-AP

Synonymes

KIAA0210, TBC1 domain family member 5, TBC1 domain family, member 5, TBC1D5



Applications testées

Résultats positifs en WBcellules HEK-293T, cellules A431, cellules Jurkat, cellules PC-3
Résultats positifs en IPcellules A431
Résultats positifs en IHCtissu testiculaire de souris,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

17078-1-AP cible TBC1D5 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène TBC1D5 Protéine recombinante Ag10991
Nom complet TBC1 domain family, member 5
Masse moléculaire calculée 795 aa, 89 kDa
Poids moléculaire observé 89 kDa
Numéro d’acquisition GenBankBC013145
Symbole du gène TBC1D5
Identification du gène (NCBI) 9779
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TBC1D5, a member of TBC (Tre2/Bub2/Cdc16)1 domain family, is a novel retromer-interacting protein. TBC1D5 is a Rab GTPase-activating proteins (GAPs) proteins that negatively regulates VPS35/29/26 recruitment and causes Rab7 to dissociate from the membrane. TBC1D5 could bridge the endosome and autophagosome via its C-terminal LIR motif, and Rab GAPs are implicated in the reprogramming of the endocytic trafficking events under starvation-induced autophagy. The TBC1D5 protein exists 89 kDa and 91 kDa isoforms.

Protocole

Product Specific Protocols
WB protocol for TBC1D5 antibody 17078-1-APDownload protocol
IHC protocol for TBC1D5 antibody 17078-1-APDownload protocol
IP protocol for TBC1D5 antibody 17078-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Sci Adv

De novo macrocyclic peptides for inhibiting, stabilizing, and probing the function of the retromer endosomal trafficking complex.

Authors - Kai-En Chen
humanWB

EMBO J

Control of RAB7 activity and localization through the retromer-TBC1D5 complex enables RAB7-dependent mitophagy.

Authors - Ana Jimenez-Orgaz
  • KO Validated
humanWB

J Cell Biol

Retromer and TBC1D5 maintain late endosomal RAB7 domains to enable amino acid-induced mTORC1 signaling.

Authors - Arunas Kvainickas
  • KO Validated
WB

EMBO Rep

BioID reveals an ATG9A interaction with ATG13-ATG101 in the degradation of p62/SQSTM1-ubiquitin clusters.

Authors - Ashari R Kannangara
humanWB

Int J Biol Sci

TBK1 Facilitates GLUT1-Dependent Glucose Consumption by suppressing mTORC1 Signaling in Colorectal Cancer Progression.

Authors - Diyuan Zhou
  • KD Validated
mouseIF

Mol Biol Cell

Trafficking defects in WASH-knockout fibroblasts originate from collapsed endosomal and lysosomal networks.

Authors - Gomez Timothy S TS

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

q (Verified Customer) (06-01-2021)

It is OK to detect TBC1D5 (100 kD) in both mouse and postmortem brain lysates, although there are some non specific bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: mouse brain and postmortembrain lysates
TBC1D5 Antibody Western Blot validation (1:3000 dilution) in mouse brain and postmortembrain lysates (Cat no:17078-1-AP)
FH

Florian (Verified Customer) (04-09-2019)

This is a very strong antibody that is reasonably specific in western blot applications. We have validated it through Crispr/Cas9 mediated knockout of TBC1D5 in HeLa cells (Jimenez-Orgaz et al, EMBOJ, 2018). While the strongest band is indeed TBC1D5, as our knockout confirms, it also produces several weaker, unspecific bands on membranes blocked with 5% milk. Because of the unspecific bands, I rate it with four stars instead of five. We have not tested it for immunofluorescence. Overall, it is a very good antibody that will probably also work at much lower dilutions than 1:1000. Diluting it further may also reduce the unspecific signal.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hela cells
FH

David (Verified Customer) (02-28-2019)

Hela cells stained with anti-TBC1D5 (green) and Hoechst (blue)

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Hela cells
TBC1D5 Antibody Western Blot,Immunofluorescence, validation (1:200 dilution) in Hela cells (Cat no:17078-1-AP)