Anticorps Polyclonal de lapin anti-TBC1D23

TBC1D23 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (2)

N° de cat : 17002-1-AP

Synonymes

NS4ATP1, TBC1 domain family member 23, TBC1 domain family, member 23, TBC1D23

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Galerie de données de validation

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  WB analysis of HepG2 using 17002-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17002-1-AP (TBC1D23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17002-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17002-1-AP (TBC1D23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 17002-1-AP

  IP result of anti-TBC1D23 (IP:17002-1-AP, 4ug; Detection:17002-1-AP 1:500) with HepG2 cells lysate 2400ug.

• 

  IHC staining of human liver using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 17002-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 17002-1-AP (TBC1D23 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu hépatique humain, tissu ovarien humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

17002-1-AP cible TBC1D23 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TBC1D23 Protéine recombinante Ag10697
• Nom complet: TBC1 domain family, member 23
• Masse moléculaire calculée: 699 aa, 78 kDa
• Poids moléculaire observé: 70-78 kDa
• Numéro d’acquisition GenBank: BC010221
• Symbole du gène: TBC1D23
• Identification du gène (NCBI): 55773
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TBC1D23, also named as NS4ATP1, is a gene down-regulated by Pneumocystis infection. Pneumocystis pneumonia is a common opportunistic disease in AIDS patients. (PMID: 20377877) Recently it has been found that TBC1D23 gene is the most frequently mutated in MSI-H cell lines and protein expressions of TBC1D23 in tumors with mutation were down regulated.(PMID: 20824714) This antibody is specific to TBC1D23.

Publications

Species	Application	Title
	WB	Nat Commun; TBC1D23 mediates Golgi-specific LKB1 signaling; Authors - Yingfeng Tu
human	WB,IF	Am J Hum Genet; Homozygous Mutations in TBC1D23 Lead to a Non-degenerative Form of Pontocerebellar Hypoplasia.; Authors - Isaac Marin-Valencia
human	IF	Am J Hum Genet; Homozygous Truncating Variants in TBC1D23 Cause Pontocerebellar Hypoplasia and Alter Cortical Development.; Authors - Ekaterina L Ivanova
human	WB,IHC	Int J Cancer; Identification of frequently mutated genes with relevance to nonsense mediated mRNA decay in the high microsatellite instability cancers.; Authors - Shin Nara N
human	IHC,WB,IF	J Cell Mol Med; TBC1 domain family member 23 interacts with Ras-related protein Rab-11A to promote poor prognosis of non-small-cell lung cancer via β1-integrin.; Authors - Yao Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christine (Verified Customer) (01-27-2023)

Main band detected below the 85 kDa marker (expected size of TBC1D23 is 78, observed is 70-78).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: HEK293T

FH

Jerome (Verified Customer) (05-22-2019)

Most cells show the expected Golgi localisation; but not all. High background

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HeLa cells

TBC1D23 Antibody Immunofluorescence, validation (1:100 dilution) in HeLa cells (Cat no:17002-1-AP)