Anticorps Polyclonal de lapin anti-TAF15

• Phare
• Validé par KD/KO

TAF15 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, RIP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 24235-1-AP

Synonymes

TAF(II)68, RNA-binding protein 56, RNA binding protein 56, RBP56, hTAFII68

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Galerie de données de validation

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  WB analysis of HEK-293 using 24235-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24235-1-AP (TAF15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24235-1-AP

  WB result of TAF15 antibody (24235-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAF15 transfected HeLa cells.

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  IHC staining of human testis using 24235-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 24235-1-AP (TAF15 Antibody) at dilution of 1:50.

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  IHC staining of human kidney using 24235-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 24235-1-AP (TAF15 Antibody) at dilution of 1:50.

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  IF Staining of HeLa using 24235-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TAF15 antibody (24235-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa
• Résultats positifs en IHC: tissu testiculaire humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

24235-1-AP cible TAF15 dans les applications de WB, IHC, IF/ICC, RIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TAF15 Protéine recombinante Ag20828
• Nom complet: TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
• Masse moléculaire calculée: 592 aa, 62 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC046099
• Symbole du gène: TAF15
• Identification du gène (NCBI): 8148
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TAF15 (TAF2N or TAFII68) is a nuclear protein known to associate with a distinct subpopulation of transcription factor IID (TFIID), a multi-subunit complex that nucleates the pre-initiation complex on promoters of on protein-coding genes. TAF15 harbors a transcriptional activation domain, a RNA recognition motif and many Arg-Gly-Gly repeats that participate in RNA binding. TAF15 along with FUS and EWS constitutes the FET (FUS/EWS/TAF15) protein family of heterogeneous nuclear ribonucleoproteins (hnRNPs) class of RNA binding proteins that are multifunctional proteins implicated in numerous aspects of RNA processing/functions. TAF15 is a nuclear protein that shuttle between cytoplasm-nucleus and as RNA/ssDNA-binding protein, it play specific roles during transcription initiation at distinct promoters and enter the preinitiation complex together with the RNA polymerase II. TAF15 as well as other FET member genes are frequently translocated in sarcomas and rare hematopoietic as well as epithelial cancers. Moreover, dominant mutations in genes coding for four hnRNPs (TDP-43, FUS/TLS, TAF15, and EWS) are found in familial and sporadic cases of ALS and a chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC).

Publications

Species	Application	Title
human	WB,RIP	Front Oncol; Long Non-Coding RNA CCAT2 Activates RAB14 and Acts as an Oncogene in Colorectal Cancer.; Authors - Dalu Wang
human,mouse	WB,IHC	Mol Cell Biochem; RNF144A-AS1 stabilizes TAF15 and promotes malignant biological behaviors of skin cutaneous melanoma; Authors - Shude Yang ; KD Validated