Anticorps Polyclonal de lapin anti-TAF1-Specific

TAF1-Specific Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 20260-1-AP

Synonymes

BA2R, CCG1, CCGS, Cell cycle gene 1 protein, DYT3, KAT4, N TAF1, NSCL2, OF, P250, TAF(II)250, TAF1, TAF1-Specific, TAF2A, TAFII 250, TAFII250, TBP associated factor 250 kDa

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Galerie de données de validation

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  WB analysis using 20260-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20260-1-AP (TAF1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20260-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20260-1-AP (TAF1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 20260-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:600 (under 40x lens).

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  IHC staining of human brain using 20260-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 20260-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human prostate cancer using 20260-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human prostate cancer using 20260-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human prostate cancer using 20260-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human prostate cancer using 20260-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:500 (under 40x lens).

• 

  IHC staining of human prostate cancer using 20260-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:600 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules Jurkat, cellules K-562, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

20260-1-AP cible TAF1-Specific dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa
• Masse moléculaire calculée: 213 kDa
• Poids moléculaire observé: 250-260 kDa, 50 kDa
• Numéro d’acquisition GenBank: NM_138923
• Symbole du gène: TAF1
• Identification du gène (NCBI): 6872
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TAF1, also named as BA2R, CCG1, CCGS, TAF2A, p250, Cell cycle gene 1 protein, TAFII250 and DYT3, belongs to the TAF1 family. TAF1 is the largest component and core scaffold of the TFIID basal transcription factor complex. It contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. TAF1 phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. TAF1 phosphorylates GTF2A1 and GTF2F1 on Ser residues. It possesses DNA-binding activity. It is essential for progression of the G1 phase of the cell cycle. Defects in TAF1 are the cause of dystonia type 3 (DYT3) which also called X-linked dystonia-parkinsonism (XDP). The antibody is specific to TAF1. It has several isoforms with predicted MW's of 200-250 kDa and 170-180 kDa, it also could get about 50 kDa band which is caused by the lysate preparation.

Publications

Species	Application	Title
human	WB	J Extracell Vesicles; Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.; Authors - Xiaoyu Yu
human	WB	J Clin Invest; Long noncoding RNA HITT coordinates with RGS2 to inhibit PD-L1 translation in T cell immunity; Authors - Qingyu Lin