Anticorps Polyclonal de lapin anti-TACC3

• Phare
• Validé par KD/KO

TACC3 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 25697-1-AP

Synonymes

ERIC 1, ERIC1, TACC3

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Galerie de données de validation

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  WB analysis using 25697-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25697-1-AP (TACC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 25697-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25697-1-AP (TACC3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 25697-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25697-1-AP (TACC3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 25697-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25697-1-AP (TACC3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 25697-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25697-1-AP (TACC3 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 25697-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TACC3 antibody (25697-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

25697-1-AP cible TACC3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TACC3 Protéine recombinante Ag22516
• Nom complet: transforming, acidic coiled-coil containing protein 3
• Masse moléculaire calculée: 838 aa, 91 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC111771
• Symbole du gène: TACC3
• Identification du gène (NCBI): 10460
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TACC3, also known as ERIC 1, belongs to the TACC family. TACC family members TACC1, TACC2, and TACC3 map very closely to the corresponding FGFR1, FGFR2, FGFR3 genes on chromosomes 8, 10, and 4. Subsequently, since they are phylogenetically related, it is proposed that TACC and FGFR have similar roles in cell growth and differentiation. It plays a role in the microtubule-dependent coupling of the nucleus and the centrosome and involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors. This antibody works well in WB, IHC and IF application.

Publications

Species	Application	Title
human	WB	Nat Commun; K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis; Authors - Xiao-Lan Zhang
human	WB,IHC	Oncotarget; TACC3 promotes colorectal cancer tumourigenesis and correlates with poor prognosis.; Authors - Yong Du ; KD Validated
human	WB	Cell Death Dis; PMID: 29358577; Authors - Zhi-Rui Lin ; KD Validated
human	WB,IF	Vet Microbiol; Transforming acidic coiled-coil containing protein 3 suppresses influenza A virus replication by impeding viral endosomal trafficking and nuclear import; Authors - Lulu Deng ; KD Validated
human,mouse	WB,IHC,IF	Cell Death Dis; Knockdown of TACC3 inhibits tumor cell proliferation and increases chemosensitivity in pancreatic cancer; Authors - Saimeng Shi ; KD Validated