Anticorps Polyclonal de lapin anti-SYNGAP1

SYNGAP1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 19739-1-AP

Synonymes

KIAA1938, MRD5, Neuronal RasGAP, RASA1, RASA5, Synaptic Ras GAP 1, SYNGAP, SYNGAP1

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Galerie de données de validation

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  WB analysis using 19739-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19739-1-AP (SYNGAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19739-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19739-1-AP (SYNGAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 19739-1-AP

  IP result of anti-SYNGAP1 (IP:19739-1-AP, 4ug; Detection:19739-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 19739-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19739-1-AP (SYNGAP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19739-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19739-1-AP (SYNGAP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-251 using 19739-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using 19739-1-AP (SYNGAP1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissus cérébraux de porc, tissus cérébraux de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U-251,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below

Informations sur le produit

19739-1-AP cible SYNGAP1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: synaptic Ras GTPase activating protein 1 homolog (rat)
• Masse moléculaire calculée: 1343 aa, 148 kDa
• Poids moléculaire observé: 148 kDa
• Numéro d’acquisition GenBank: NM_006772
• Symbole du gène: SYNGAP1
• Identification du gène (NCBI): 8831
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SYNGAP1, also named as KIAA1938, is the major constituent of the PSD essential for postsynaptic signaling. It's an inhibitory regulator of the Ras-cAMP pathway. SYNGAP1 is a member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. SYNGAP1 regulates AMPAR-mediated miniature excitatory postsynaptic currents. SYNGAP1 may be involved in certain forms of brain injury, leading to long-term learning and memory deficits Defects in SYNGAP1 are the cause of mental retardation autosomal dominant type 5 (MRD5).

Publications

Species	Application	Title
mouse	WB	Front Aging Neurosci; Impaired Learning and Memory Ability Induced by a Bilaterally Hippocampal Injection of Streptozotocin in Mice: Involved With the Adaptive Changes of Synaptic Plasticity.; Authors - Cong-Cong Qi
mouse	WB	Neurochem Int; Resveratrol ameliorates learning and memory impairments induced by bilateral hippocampal injection of streptozotocin in mice.; Authors - Ya-Dong Wei
mouse	WB,IHC	Eur J Neurosci; SynGAP is expressed in the murine suprachiasmatic nucleus and regulates circadian-gated locomotor activity and light-entrainment capacity.; Authors - Sydney Aten
mouse	WB	Neuroscience; Further Studies on the Role of BTBD9 in the Cerebellum, Sleep-like Behaviors and the Restless Legs Syndrome; Authors - Shangru Lyu
mouse	WB	Mol Neurobiol; SNX17 Mediates Dendritic Spine Maturation via p140Cap; Authors - Qiuyan Cui
mouse	WB	J Nutr Biochem; Hippocampal proteomic changes in high-fat diet-induced obese mice associated with memory decline; Authors - Ping Lu