Anticorps Polyclonal de lapin anti-SYNCRIP

• Phare
• Validé par KD/KO

SYNCRIP Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 14024-1-AP

Synonymes

GRY RBP, hnRNP Q, HNRPQ, HNRPQ1, NS1 associated protein 1, NSAP1, pp68, RP1 3J17.2, SYNCRIP

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Galerie de données de validation

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  WB analysis of A431 using 14024-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14024-1-AP (SYNCRIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14024-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14024-1-AP (SYNCRIP antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14024-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14024-1-AP (SYNCRIP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14024-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14024-1-AP (SYNCRIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14024-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14024-1-AP (SYNCRIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 14024-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14024-1-AP (SYNCRIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 14024-1-AP

  IP result of anti-SYNCRIP (IP:14024-1-AP, 4ug; Detection:14024-1-AP 1:500) with A431 cells lysate 1200ug.

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  IHC staining of human colon cancer using 14024-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14024-1-AP (SYNCRIP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14024-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14024-1-AP (SYNCRIP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 14024-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SYNCRIP antibody (14024-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). DAPI (blue).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules Jurkat
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

14024-1-AP cible SYNCRIP dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SYNCRIP Protéine recombinante Ag5167
• Nom complet: synaptotagmin binding, cytoplasmic RNA interacting protein
• Masse moléculaire calculée: 623 aa, 70 kDa
• Poids moléculaire observé: 64-74 kDa
• Numéro d’acquisition GenBank: BC032643
• Symbole du gène: SYNCRIP
• Identification du gène (NCBI): 10492
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Synaptotagmin-binding, cytoplasmic RNA-interacting protein(SYNCRIP), also identified as hnRNP Q, involves in mRNA processing mechanisms. It's component of the CRD-mediated complex that promotes MYC mRNA stability. Also it is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. As previously reported, the 65 and 74 kDa proteins corresponded to two isoforms of SYNCRIP produced from alternatively spliced mRNAs (PMID: 15340051).

Publications

Species	Application	Title
human	WB,IP	Nucleic Acids Res; Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.; Authors - Evan P Booy
mouse	WB	Sci Rep; Identification of RNA-binding proteins that partner with Lin28a to regulate Dnmt3a expression.; Authors - Silvia Parisi ; KD Validated