Anticorps Polyclonal de lapin anti-SYAP1

• Phare
• Validé par KD/KO

SYAP1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 16272-1-AP

Synonymes

PRO3113, SYAP1, Synapse associated protein 1

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Galerie de données de validation

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  WB analysis using 16272-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16272-1-AP (SYAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16272-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16272-1-AP (SYAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 16272-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16272-1-AP (SYAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 16272-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16272-1-AP (SYAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 16272-1-AP

  IP result of anti-SYAP1 (IP:16272-1-AP, 4ug; Detection:16272-1-AP 1:500) with L02 cells lysate 1800ug.

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  IHC staining of human cervical cancer using 16272-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16272-1-AP (SYAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 16272-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16272-1-AP (SYAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 16272-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16272-1-AP (SYAP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 16272-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16272-1-AP (SYAP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 16272-1-AP

  Immunofluorescent analysis of HepG2 cells using 16272-1-AP (SYAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 16272-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SYAP1 antibody (16272-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 16272-1-AP

  Immunofluorescent analysis of A431 cells using 16272-1-AP (SYAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A431, cellules HepG2, cellules L02, cellules SMMC-7721, tissu hépatique de souris, tissu pulmonaire de rat, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

16272-1-AP cible SYAP1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SYAP1 Protéine recombinante Ag9342
• Nom complet: synapse associated protein 1, SAP47 homolog (Drosophila)
• Masse moléculaire calculée: 352 aa, 40 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC014657
• Symbole du gène: SYAP1
• Identification du gène (NCBI): 94056
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SYAP1(Synapse-associated protein 1) is a human homologue of the Drosophila SAP47 (synapse associated protein), which is recognized by a monoclonal antibody that selectively stain synaptic terminals.This protein is a 352 amino acid protein that is ubiquitously expressed in adult tissues. SYAP1 contains one BSD domain which is is an approximately 60 amino acid long protein domain named after the BTF2-like transcription factors, Synapse-associated proteins and DOS2-like proteins in which it is found.

Publications

Species	Application	Title
mouse	WB	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
mouse	WB,IF	Histochem Cell Biol; Initial characterization of a Syap1 knock-out mouse and distribution of Syap1 in mouse brain and cultured motoneurons.; Authors - Dominique Schmitt ; KO Validated
mouse	IF	Biol Open; An essential role of the mouse synapse-associated protein Syap1 in circuits for spontaneous motor activity and rotarod balance.; Authors - Cora R von Collenberg