• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-SUV39H1

SUV39H1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 10574-1-AP

Synonymes

H3 K9 HMTase 1, KMT1A, Lysine N methyltransferase 1A, MG44, Su(var)3 9 homolog 1, SUV39H, SUV39H1



Applications testées

Résultats positifs en WBcellules HCT 116, cellules C6, cellules HEK-293, cellules HeLa, cellules HepG2, cellules NIH/3T3, tissu d'intestin grêle de souris
Résultats positifs en IPcellules HeLa

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

10574-1-AP cible SUV39H1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène SUV39H1 Protéine recombinante Ag0854
Nom complet suppressor of variegation 3-9 homolog 1 (Drosophila)
Masse moléculaire calculée 48 kDa
Poids moléculaire observé 48-50 kDa
Numéro d’acquisition GenBankBC006238
Symbole du gène SUV39H1
Identification du gène (NCBI) 6839
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histone-lysine N-methyltransferase (SUV39H1), a nuclear protein, moves to the centromeres during mitosis and specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. It plays a vital role in heterochromatin organization, chromosome segregation, and mitotic progression. SUV39H1 has 2 isoforms with molecular weights of 48 and 49 kDa.

Protocole

Product Specific Protocols
WB protocol for SUV39H1 antibody 10574-1-APDownload protocol
IP protocol for SUV39H1 antibody 10574-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Nucleic Acids Res

ATM, KAP1 and the Epstein-Barr virus polymerase processivity factor direct traffic at the intersection of transcription and replication

Authors - Huanzhou Xu
mouseWB

Nat Commun

The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.

Authors - Guang Yang
  • KO Validated
humanWB

J Med Chem

Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis.

Authors - Ting Peng
humanWB

Cell Death Dis

Dicer promotes tumorigenesis by translocating to nucleus to promote SFRP1 promoter methylation in cholangiocarcinoma cells.

Authors - Wenlong Cheng
humanWB

Oncotarget

Downregulation of HP1α suppresses proliferation of cholangiocarcinoma by restoring SFRP1 expression.

Authors - Wenlong Cheng
humanWB

iScience

Linkage of methionine addiction, histone lysine hypermethylation, and malignancy.

Authors - Jun Yamamoto

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Roy (Verified Customer) (06-15-2024)

Great antibody for detecting SUV39H1 from HeLa extracts (1/1000 dilution, overnight incubation at 4°C)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa