Anticorps Polyclonal de lapin anti-SUPV3L1

SUPV3L1 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 12826-1-AP

Synonymes

SUPV3L1, SUV3, SUV3 like protein 1

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Galerie de données de validation

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  WB analysis using 12826-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 12826-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12826-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 12826-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa cells using 12826-1-AP

  HeLa cell were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 Antibody) at dilution of 1:500.

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  IP experiment of HeLa using 12826-1-AP

  IP result of anti-SUPV3L1 (IP:12826-1-AP, 4ug; Detection:12826-1-AP 1:300) with HeLa cells lysate 1200ug.

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  IP experiment of HeLa using 12826-1-AP

  IP result of anti-SUPV3L1 (IP:12826-1-AP, 4ug; Detection:12826-1-AP 1:300) with HeLa cells lysate 1200ug.

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  IP experiment of HeLa using 12826-1-AP

  IP result of anti-SUPV3L1 (IP:12826-1-AP, 4ug; Detection:12826-1-AP 1:300) with HeLa cells lysate 1200ug.

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  IF Staining of HeLa using 12826-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12826-1-AP (SUPV3L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules PC-3
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

12826-1-AP cible SUPV3L1 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SUPV3L1 Protéine recombinante Ag3542
• Nom complet: suppressor of var1, 3-like 1 (S. cerevisiae)
• Masse moléculaire calculée: 786 aa, 88 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC036112
• Symbole du gène: SUPV3L1
• Identification du gène (NCBI): 6832
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SUPV3L1 is a major helicase player in mitochondrial RNA metabolism. It is involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules (PMID: 29967381).

Publications

Species	Application	Title
human	WB	J Inherit Metab Dis; Mitochondrial RNA processing defect caused by a SUPV3L1 mutation in two siblings with a novel neurodegenerative syndrome.; Authors - Selma L van Esveld
human	WB	Biochimie; Cathepsin D inhibition during neuronal differentiation selectively affects individual proteins instead of overall protein turnover; Authors - Johannes Schneider