Anticorps Polyclonal de lapin anti-SUPT16H

SUPT16H Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 20551-1-AP

Synonymes

CDC68, FACT 140 kDa subunit, FACT complex subunit SPT16, FACT140, FACTP140, hSPT16, SPT16/CDC68, SUPT16H

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Galerie de données de validation

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  WB analysis of mouse brain using 20551-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20551-1-AP (SUPT16H antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20551-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20551-1-AP (SUPT16H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20551-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20551-1-AP (SUPT16H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 20551-1-AP

  IP result of anti-SUPT16H (IP:20551-1-AP, 4ug; Detection:20551-1-AP 1:1000) with mouse brain tissue lysate 3440ug.

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  IHC staining of human tonsillitis using 20551-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 20551-1-AP (SUPT16H antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human tonsillitis using 20551-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 20551-1-AP (SUPT16H antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human tonsillitis using 20551-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 20551-1-AP (SUPT16H antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human tonsillitis using 20551-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 20551-1-AP (SUPT16H antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human spleen using 20551-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20551-1-AP (SUPT16H antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 20551-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20551-1-AP (SUPT16H antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 20551-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20551-1-AP (SUPT16H antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 20551-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20551-1-AP (SUPT16H antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris,
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 2 publications below

Informations sur le produit

20551-1-AP cible SUPT16H dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: suppressor of Ty 16 homolog (S. cerevisiae)
• Masse moléculaire calculée: 120 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: NM_007192
• Symbole du gène: SUPT16H
• Identification du gène (NCBI): 11198
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SUPT16H, also named as FACT140, FACTP140, SPT16 and CDC68, belongs to the peptidase M24 family and SPT16 subfamily. SUPT16H is a component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). It also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. The antibody is specific to SUPT16H.

Publications

Species	Application	Title
		Arterioscler Thromb Vasc Biol; Novel Long Noncoding RNA, Macrophage Inflammation-Suppressing Transcript (MIST), Regulates Macrophage Activation During Obesity.; Authors - Kenneth Stapleton
mouse	IHC	FASEB J; Chronic exercise training activates histone turnover in mouse skeletal muscle fibers.; Authors - Ikumi Ohsawa
human	WB	J Mol Biol; Proteomic characterization of the nucleolar linker histone H1 interaction network.; Authors - Heather J Szerlong
human	WB,IHC	Biomed Pharmacother; CBL0137 activates ROS/BAX signaling to promote caspase-3/GSDME-dependent pyroptosis in ovarian cancer cells; Authors - Chang Yang