Anticorps Polyclonal de lapin anti-SUMO2/3

• Phare
• Validé par KD/KO

SUMO2/3 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (2)

N° de cat : 11251-1-AP

Synonymes

HSMT3, Sentrin 2, SMT3 homolog 2, SMT3B, SMT3H2, SUMO 2, SUMO 3, SUMO2, SUMO2/3, SUMO3, Ubiquitin like protein SMT3B

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Galerie de données de validation

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  WB analysis of HEK-293 using 11251-1-AP

  WB result of SUMO2/3 antibody (11251-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUMO2/3 transfected HEK-293 cells.

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  WB analysis using 11251-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11251-1-AP (SUMO2/3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 11251-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11251-1-AP (SUMO2/3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 11251-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SUMO2/3 antibody (11251-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules L02, cellules PC-12
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

11251-1-AP cible SUMO2/3 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SUMO2/3 Protéine recombinante Ag1778
• Nom complet: SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
• Masse moléculaire calculée: 11 kDa
• Poids moléculaire observé: 11-20 kDa
• Numéro d’acquisition GenBank: BC016775
• Symbole du gène: SUMO2
• Identification du gène (NCBI): 6613
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.

Publications

Species	Application	Title
human	WB	Sci Adv; XAF1 promotes anti-RNA virus immune responses by regulating chromatin accessibility; Authors - Ming Kuang
human	WB	Theranostics; Chlorogenic acid effectively treats cancers through induction of cancer cell differentiation.; Authors - Shuai Huang
human	IP	mSystems; Proteomic Profiling Identifies Kaposi's Sarcoma-Associated Herpesvirus (KSHV)-Encoded LANASIM-Associated Proteins in Hypoxia.; Authors - Xiaoqing Liu
mouse	WB	iScience; Alternative transcribed 3' isoform of long non-coding RNA Malat1 inhibits mouse retinal oxidative stress; Authors - Amr R Ghanam
mouse	IP	Int J Mol Med; SUMO2 modification of Aurora B and its impact on follicular development and atresia in the mouse ovary.; Authors - Jing Cao
mouse	WB,IP	Aging (Albany NY); SUMO1-regulated DBC1 promotes p53-dependent stress-induced apoptosis of lens epithelial cells; Authors - Yan Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Verdiana (Verified Customer) (03-02-2023)

Good bands. Little aspecific I think but the gels we use have a high resolution and also those were lympoblastoid cells and we know they can me strange. Anyway, it is a good antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Lymphoblastic cell line

SUMO2/3 Antibody Western Blot validation (1:1000 dilution) in Lymphoblastic cell line (Cat no:11251-1-AP)

FH

Shubham (Verified Customer) (03-13-2019)

Good

• Applications: Western Blot,
• Cell Tissue Type: AC16

SUMO2/3 Antibody Western Blot, validation ( dilution) in AC16 (Cat no:11251-1-AP)