Anticorps Polyclonal de lapin anti-SUMO2/3

• Phare
• Validé par KD/KO

SUMO2/3 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 10947-1-AP

Synonymes

SUMO2, SUMO2/3

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Galerie de données de validation

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  WB analysis of HEK-293 using 10947-1-AP

  WB result of SUMO2/3 antibody (10947-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUMO2/3 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 10947-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10947-1-AP (SUMO2/3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10947-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10947-1-AP (SUMO2/3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10947-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10947-1-AP (SUMO2/3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Recombinant protein using 10947-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 10947-1-AP (SUMO2/3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10947-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10947-1-AP (SUMO2/3 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HeLa using 10947-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUMO2/3 antibody (10947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, cellules Jurkat, protéine protéine recombinante
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

10947-1-AP cible SUMO2/3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SUMO2/3 Protéine recombinante Ag1388
• Nom complet: SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
• Masse moléculaire calculée: 11 kDa
• Poids moléculaire observé: 18-20 kDa
• Numéro d’acquisition GenBank: BC008450
• Symbole du gène: SUMO2
• Identification du gène (NCBI): 6613
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.

Publications

Species	Application	Title
human	WB,IF	Cell Death Discov; Loss of PML nuclear bodies in familial amyotrophic lateral sclerosis-frontotemporal dementia; Authors - Francesco Antoniani