Anticorps Polyclonal de lapin anti-SULT4A1

• Phare
• Validé par KD/KO

SULT4A1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 12578-1-AP

Synonymes

BR STL 1, BRSTL1, hBR STL, hBR STL 1, NST, ST4A1, Sulfotransferase 4A1, SULT4A1, SULTX3

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Galerie de données de validation

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  WB analysis using 12578-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12578-1-AP (SULT4A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12578-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12578-1-AP (SULT4A1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12578-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12578-1-AP (SULT4A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12578-1-AP

  IP result of anti-SULT4A1 (IP:12578-1-AP, 4ug; Detection:12578-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cerveau de rat, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Informations sur le produit

12578-1-AP cible SULT4A1 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SULT4A1 Protéine recombinante Ag3271
• Nom complet: sulfotransferase family 4A, member 1
• Masse moléculaire calculée: 284 aa, 33 kDa
• Poids moléculaire observé: 30-33 kDa
• Numéro d’acquisition GenBank: BC022459
• Symbole du gène: SULT4A1
• Identification du gène (NCBI): 25830
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SULT4A1, also named as SULTX3, NST, ST4A1, hBR-STL and hBR-STL-1, belongs to the sulfotransferase 1 family. It may catalyze the sulfate conjugation of many drugs, xenobiotic compounds, hormones, and neurotransmitters. SULT4A1 displays activity towards L-triiodothyronine, thyroxine, estrone, p-nitrophenol, 2-naphtylamine, and 2-naphtol. It may have a role in the sulfation of drugs and neurotransmitters in the CNS. SULT4A1 is a brain-specific sulfotransferase believed to be involved in the metabolism of neurotransmitters. It is a novel cytosolic sulfotransferase that is primarily expressed in the brain.

Publications

Species	Application	Title
mouse,rat	WB,IF	J Neurosci; SULT4A1 Modulates Synaptic Development and Function by Promoting the Formation of PSD-95/NMDAR Complex.; Authors - Lorenza Culotta ; KD Validated
human,rat	WB,IHC	Biochem Pharmacol; Identification of metabolic pattern and bioactive form of resveratrol in human medulloblastoma cells.; Authors - Shu Xiao-Hong XH
mouse	WB	Mol Pharmacol; Regulation of mouse brain-selective sulfotransferase sult4a1 by cAMP response element-binding protein and activating transcription factor-2.; Authors - Butcher Neville J NJ
human	WB	FEBS J; Distinct sulfonation activities in resveratrol-sensitive and resveratrol-insensitive human glioblastoma cells.; Authors - Sun Zheng Z
	WB	Sci Rep; Sulfotransferase 4A1 activity facilitates sulfate-dependent cellular protection to oxidative stress.; Authors - Evan J Brettrager
human	WB,IHC,IF	PLoS One; Metabolic patterns and biotransformation activities of resveratrol in human glioblastoma cells: relevance with therapeutic efficacies.; Authors - Shu Xiao-Hong XH