Anticorps Polyclonal de lapin anti-SULT2B1

SULT2B1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 12879-1-AP

Synonymes

Alcohol sulfotransferase, HSST2, ST2B1, Sulfotransferase 2B1, SULT2B1, sult2b1a, sult2b1b

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Galerie de données de validation

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  WB analysis of mouse skin using 12879-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 12879-1-AP (SULT2B1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skin using 12879-1-AP

  IP result of anti-SULT2B1 (IP:12879-1-AP, 4ug; Detection:12879-1-AP 1:400) with mouse skin tissue lysate 3200ug.

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  IHC staining of human prostate cancer using 12879-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 12879-1-AP (SULT2B1 Antibody) at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

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  IHC staining of human prostate cancer using 12879-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 12879-1-AP (SULT2B1 Antibody) at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

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  IHC staining of human prostate cancer using 12879-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12879-1-AP (SULT2B1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 12879-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 12879-1-AP (SULT2B1 Antibody) at dilution of 1:50.

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  IHC staining of human placenta using 12879-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 12879-1-AP (SULT2B1 Antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: tissu cutané de souris
• Résultats positifs en IP: tissu cutané de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

12879-1-AP cible SULT2B1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SULT2B1 Protéine recombinante Ag3838
• Nom complet: sulfotransferase family, cytosolic, 2B, member 1
• Masse moléculaire calculée: 365 aa, 41 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC034694
• Symbole du gène: SULT2B1
• Identification du gène (NCBI): 6820
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SULT2B1 is a member of hydroxysteroid sulfotransferase (SULT) family that catalyzes sulfonation of hormones and other molecules. SULT2B1 encodes two isoforms, SULT2B1a and SULT2B1b, that differ only at their amino termini but have distinct biochemical properties as well as tissue distribution. The human fetal brain appears to only express the SULT2B1a isoform, while SULT2B1b is the predominant hydroxysteroid sulfotransferase expressed in human skin. Northern blot analysis revealed SULT2B1a has a limited tissue distribution in liver, adrenal and stomach, whereas SULT2B1b can be detected in a variety of hormone-responsive tissues including placenta, ovary, uterus, and prostate. This antibody recognizes both of ULT2B1a and SULT2B1b.

Publications

Species	Application	Title
human	WB	J Lipid Res; Activation of liver X receptor plays a central role in antiviral actions of 25-hydroxycholesterol.; Authors - Ying Liu
mouse	WB	Drug Metab Dispos; Sulfotransferase 4A1 (SULT4A1) increases its expression in mouse neurons as they mature.; Authors - Takuyu Hashiguchi