Anticorps Polyclonal de lapin anti-SUB1

• Phare
• Validé par KD/KO

SUB1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 10948-2-AP

Synonymes

p14, PC4, Positive cofactor 4, RPO2TC1, SUB1, SUB1 homolog, SUB1 homolog (S. cerevisiae)

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Galerie de données de validation

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  WB analysis of A549 using 10948-2-AP

  WB result of SUB1 antibody (10948-2-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUB1 transfected A549 cells.

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  WB analysis of HeLa using 10948-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10948-2-AP (SUB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10948-2-AP

  WB result of SUB1 antibody (10948-2-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUB1 transfected HeLa cells.

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  WB analysis using 10948-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10948-2-AP (SUB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10948-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10948-2-AP (SUB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10948-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10948-2-AP (SUB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10948-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10948-2-AP (SUB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 10948-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10948-2-AP (SUB1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10948-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10948-2-AP (SUB1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 10948-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10948-2-AP (SUB1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 10948-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10948-2-AP (SUB1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10948-2-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10948-2-AP (SUB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

10948-2-AP cible SUB1 dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SUB1 Protéine recombinante Ag1390
• Nom complet: SUB1 homolog (S. cerevisiae)
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 18-23 kDa
• Numéro d’acquisition GenBank: BC009610
• Symbole du gène: SUB1
• Identification du gène (NCBI): 10923
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SUB1 gene encodes activated RNA polymerase II transcriptional coactivator p15, also termed as positive cofactor 4 (PC4) or p14. SUB1 is a sort of general coactivator that functions in assembling and stablization of transcription complex. It has DNA binding activity and is localized in the nucleus, mulitple modification sites of phosphorylation or acetylation may turn it appear slightly shifted molecular weight. Catalog#10948-2-AP is a rabbit polyclonal antibody raised against full-length human SUB1. This antibody recognize 14-16 kDa (P14 or P15), 20 kDa(phospho form) and 30 kDa (Dimer form).

Publications

Species	Application	Title
mouse	WB,IHC	J Proteome Res; Novel breast cancer metastasis-associated proteins.; Authors - Ho Jiapei J
mouse	WB	J Cell Biochem; Knockdown of nucleosome assembly protein 1-like 1 promotes dimethyl sulfoxide-induced differentiation of P19CL6 cells into cardiomyocytes.; Authors - Li Lu L
human,mouse	WB,IHC,IF,CoIP	Sci China Life Sci; SUB1 promotes colorectal cancer metastasis by activating NF-κB signaling via UBR5-mediated ubiquitination of UBXN1; Authors - Hao Wang ; KO Validated