Anticorps Polyclonal de lapin anti-STT3B

• Phare
• Validé par KD/KO

STT3B Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 15323-1-AP

Synonymes

SIMP, STT3 B, STT3B

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Galerie de données de validation

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  WB analysis using 15323-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15323-1-AP (STT3B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15323-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15323-1-AP (STT3B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15323-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15323-1-AP (STT3B Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 15323-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15323-1-AP (STT3B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 15323-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15323-1-AP (STT3B Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 15323-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15323-1-AP (STT3B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 15323-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15323-1-AP (STT3B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 15323-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15323-1-AP (STT3B Antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of HepG2 using 15323-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15323-1-AP (STT3B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, tissu cérébral humain, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

15323-1-AP cible STT3B dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STT3B Protéine recombinante Ag2039
• Nom complet: STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae)
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 94 kDa
• Numéro d’acquisition GenBank: BC015880
• Symbole du gène: STT3B
• Identification du gène (NCBI): 201595
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STT3B is a component of the N-oligosaccharyl transferase (OST) enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3B seems to be involved in complex substrate specificity

Publications

Species	Application	Title
human	WB	Nat Chem Biol; Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells.; Authors - Cecilia Lopez-Sambrooks
human	WB	J Cachexia Sarcopenia Muscle; Comparative proteomic analyses of Duchenne muscular dystrophy and Becker muscular dystrophy muscles: changes contributing to preserve muscle function in Becker muscular dystrophy patients.; Authors - Daniele Capitanio
human	WB,CoIP	Mol Cell Proteomics; Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis; Authors - Madison T. Wright
human	WB	MBio; Dengue Virus Hijacks a Noncanonical Oxidoreductase Function of a Cellular Oligosaccharyltransferase Complex.; Authors - David L Lin
human	WB	EBioMedicine; Targeting conserved N-glycosylation blocks SARS-CoV-2 variant infection in vitro.; Authors - Hsiang-Chi Huang
human	WB	Cell Chem Biol; Editing N-Glycan Site Occupancy with Small-Molecule Oligosaccharyltransferase Inhibitors.; Authors - Natalia Rinis ; KO Validated