- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-STOML2
STOML2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugaison
Non conjugué
N° de cat : 10348-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules C6, cellules HEK-293, tissu cérébral de rat, tissu cérébral de souris |
Résultats positifs en IP | tissu cérébral de souris |
Résultats positifs en IHC | tissu de cancer du poumon humain, tissu de cancer de l'endomètre humain, tissu de cancer du sein humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en IF/ICC | cellules HeLa, cellules MCF-7 |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochimie (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 7 publications below |
WB | See 38 publications below |
IHC | See 24 publications below |
IF | See 5 publications below |
IP | See 2 publications below |
Informations sur le produit
10348-1-AP cible STOML2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | STOML2 Protéine recombinante Ag0363 |
Nom complet | stomatin (EPB72)-like 2 |
Masse moléculaire calculée | 356 aa, 39 kDa |
Poids moléculaire observé | 39 kDa |
Numéro d’acquisition GenBank | BC002442 |
Symbole du gène | STOML2 |
Identification du gène (NCBI) | 30968 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
STOML2 (Stomatin-like protein 2; also known as SLP-2) is a widely expressed mitochondrial member of the highly conserved family of stomatin proteins. STOML2 is localized mostly in mitochondrial inner membrane, minor in plasma membrane. Human STOML2 interacts with prohibitins and regulates mitochondrial biogenesis and function (21746876). In addition, plenty of studies revealed that STOML2 was overexpressed in many human cancer tissues, and its expression may be a new valuable prognostic biomarker (19839737, 21960069). Recently STOML2 has been identified as a serological biomarker for early colorectal cancer (CRC) diagnosis (21209152). Two isoforms of STOML2 exist due to the alternative splicing, with MW of 39 kDa and 33 kDa, respectively.
Protocole
Product Specific Protocols | |
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WB protocol for STOML2 antibody 10348-1-AP | Download protocol |
IHC protocol for STOML2 antibody 10348-1-AP | Download protocol |
IF protocol for STOML2 antibody 10348-1-AP | Download protocol |
IP protocol for STOML2 antibody 10348-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cell Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria | ||
Cell Metab DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling. | ||
Clin Cancer Res Stomatin-like protein 2 is overexpressed in cancer and involved in regulating cell growth and cell adhesion in human esophageal squamous cell carcinoma. | ||
EMBO Rep The membrane scaffold SLP2 anchors a proteolytic hub in mitochondria containing PARL and the i-AAA protease YME1L. | ||
Hum Mol Genet Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation. | ||
J Pathol Knockdown of stomatin-like protein 2 (STOML2) reduces the invasive ability of glioma cells through inhibition of the NF-κB/MMP-9 pathway. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Gaurav (Verified Customer) (02-15-2024) | Good antibody which works as per the description. Detects the endogenous proteins without any problem.
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FH Hussein (Verified Customer) (03-23-2021) | I validated the antibody specificity using different siRNAs. The signal is strong in IB and bright in IF and IHC.
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FH SITING (Verified Customer) (08-31-2020) | excellent antibody
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