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  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-STOML2

STOML2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 10348-1-AP

Synonymes

EPB72 like protein 2, HSPC108, SLP 2, SLP2, stomatin (EPB72) like 2, Stomatin like protein 2, STOML2



Applications testées

Résultats positifs en WBcellules C6, cellules HEK-293, tissu cérébral de rat, tissu cérébral de souris
Résultats positifs en IPtissu cérébral de souris
Résultats positifs en IHCtissu de cancer du poumon humain, tissu de cancer de l'endomètre humain, tissu de cancer du sein humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules HeLa, cellules MCF-7

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

10348-1-AP cible STOML2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène STOML2 Protéine recombinante Ag0363
Nom complet stomatin (EPB72)-like 2
Masse moléculaire calculée 356 aa, 39 kDa
Poids moléculaire observé 39 kDa
Numéro d’acquisition GenBankBC002442
Symbole du gène STOML2
Identification du gène (NCBI) 30968
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STOML2 (Stomatin-like protein 2; also known as SLP-2) is a widely expressed mitochondrial member of the highly conserved family of stomatin proteins. STOML2 is localized mostly in mitochondrial inner membrane, minor in plasma membrane. Human STOML2 interacts with prohibitins and regulates mitochondrial biogenesis and function (21746876). In addition, plenty of studies revealed that STOML2 was overexpressed in many human cancer tissues, and its expression may be a new valuable prognostic biomarker (19839737, 21960069). Recently STOML2 has been identified as a serological biomarker for early colorectal cancer (CRC) diagnosis (21209152). Two isoforms of STOML2 exist due to the alternative splicing, with MW of 39 kDa and 33 kDa, respectively.

Protocole

Product Specific Protocols
WB protocol for STOML2 antibody 10348-1-APDownload protocol
IHC protocol for STOML2 antibody 10348-1-APDownload protocol
IF protocol for STOML2 antibody 10348-1-APDownload protocol
IP protocol for STOML2 antibody 10348-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria

Authors - Sabine Poerschke
humanWB

Cell Metab

DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.

Authors - Ricarda Richter-Dennerlein
humanWB,IHC

Clin Cancer Res

Stomatin-like protein 2 is overexpressed in cancer and involved in regulating cell growth and cell adhesion in human esophageal squamous cell carcinoma.

Authors - Zhang Liyong L
humanWB

EMBO Rep

The membrane scaffold SLP2 anchors a proteolytic hub in mitochondria containing PARL and the i-AAA protease YME1L.

Authors - Timothy Wai
humanWB

Hum Mol Genet

Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation.

Authors - McCray Brett A BA
humanWB

J Pathol

Knockdown of stomatin-like protein 2 (STOML2) reduces the invasive ability of glioma cells through inhibition of the NF-κB/MMP-9 pathway.

Authors - Song Libing L

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Gaurav (Verified Customer) (02-15-2024)

Good antibody which works as per the description. Detects the endogenous proteins without any problem.

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: HEK293, Smooth Muscle Cells
STOML2 Antibody Western Blot,Immunoprecipitation validation (1:3000 dilution) in HEK293, Smooth Muscle Cells (Cat no:10348-1-AP)
FH

Hussein (Verified Customer) (03-23-2021)

I validated the antibody specificity using different siRNAs. The signal is strong in IB and bright in IF and IHC.

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
  • Primary Antibody Dilution: WB 1:1000 (each dilution was used 3 times); IP 1:200; IHC 1:400
  • Cell Tissue Type: Breast cancer cell lines: MDA-MB-231, MDA-MB-361, BT-474, JIMT-1
FH

SITING (Verified Customer) (08-31-2020)

excellent antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: heart
STOML2 Antibody Western Blot validation (1:1000 dilution) in heart (Cat no:10348-1-AP)