Anticorps Polyclonal de lapin anti-Stomatin

Stomatin Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 12046-1-AP

Synonymes

BND7, EPB7, EPB72, Protein 7.2b, STOM, stomatin

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Galerie de données de validation

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  WB analysis of HeLa using 12046-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12046-1-AP (Stomatin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12046-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12046-1-AP (STOM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12046-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12046-1-AP (Stomatin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12046-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12046-1-AP (STOM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12046-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12046-1-AP (STOM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12046-1-AP

  IP result of anti-Stomatin (IP:12046-1-AP, 3ug; Detection:12046-1-AP 1:300) with HeLa cells lysate 2800ug.

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  IHC staining of human lung cancer using 12046-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12046-1-AP (Stomatin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12046-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12046-1-AP (Stomatin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 12046-1-AP

  Immunofluorescent analysis of A549 cells using 12046-1-AP (STOM antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules K-562, tissu hépatique humain, tissu pulmonaire humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

12046-1-AP cible Stomatin dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Stomatin Protéine recombinante Ag2676
• Nom complet: stomatin
• Masse moléculaire calculée: 288 aa, 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC010703
• Symbole du gène: Stomatin
• Identification du gène (NCBI): 2040
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STOM, also named as BND7, EPB72, Stomatin and Protein 7.2b, belongs to the band 7/mec-2 family. It is thought to regulate cation conductance. STOM may regulate ACCN1 and ACCN3 gating. STOM is a membrane protein involved in regulation of monovalent cation transport through lipid membranes. Interestingly, STOM (which has a structure similar to caveolin) is a major lipid-raft component of erythrocytes. It may have a role in membrane microdomains modulation leading to membrane budding and MPs release.(PMID:19138258)

Publications

Species	Application	Title
human	WB	Front Med; Facile discovery of red blood cell deformation and compromised membrane/skeleton assembly in Prader-Willi syndrome; Authors - Yashuang Yang
human	WB	Vox Sang; Microparticles in stored red blood cells: an approach using flow cytometry and proteomic tools.; Authors - Rubin O O
human	IHC	Chin J Cancer Res; Stomatin plays a suppressor role in non-small cell lung cancer metastasis.; Authors - Huaying An
human	WB,CoIP	Biophys J; Interaction with stomatin directs human proton channels into cholesterol-dependent membrane domains; Authors - Artem G Ayuyan