Anticorps Polyclonal de lapin anti-STMN4

STMN4 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 12027-1-AP

Synonymes

RB3, Stathmin 4, stathmin like 4, Stathmin like protein B3, STMN4

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Galerie de données de validation

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  WB analysis of human brain using 12027-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12027-1-AP (STMN4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 12027-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12027-1-AP (STMN4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12027-1-AP

  IP result of anti-STMN4 (IP:12027-1-AP, 3ug; Detection:12027-1-AP 1:1000) with mouse brain tissue lysate 6000ug.

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  IHC staining of human gliomas using 12027-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12027-1-AP (STMN4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 12027-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12027-1-AP (STMN4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 12027-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12027-1-AP (STMN4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human gliomas using 12027-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12027-1-AP (STMN4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 12027-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12027-1-AP (STMN4 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

12027-1-AP cible STMN4 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STMN4 Protéine recombinante Ag2657
• Nom complet: stathmin-like 4
• Masse moléculaire calculée: 216 aa, 25 kDa
• Poids moléculaire observé: 22-30 kDa
• Numéro d’acquisition GenBank: BC011520
• Symbole du gène: STMN4
• Identification du gène (NCBI): 81551
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RB3 (Stathmin-4), encoded by STMN4 gene, is a neuron-specific homologue of the SCG10/stathmin family proteins, possessing a unique N-terminal membrane-associated domain and the stathmin-like domain at the C terminus, which promotes microtubule (MT) catastrophe and/or tubulin sequestering. Stathmin is a ubiquitous cytosolic phosphoprotein, preferentially expressed in the nervous system, and described as a relay integrating diverse intracellular signaling pathways. RB3 is consistently up-regulated in differentiated neuroblastoma cells, and stable expression of transfected RB3 could induce differentiation, suggesting a role of RB3 during neuroblastoma differentiation. Moreover, increased RB3 is a unique response protein in the stathmin-related proteins following optical nerve axotomy and RB3 may be a critical target for neurodegenerative diseases. STMN4 has 4 isoforms with MW 25, 23, 22 and 19 kDa (refer to UniProt). Another 30 kDa isoform can be found by NCBI Protein Blast. (https://www.ncbi.nlm.nih.gov/protein/XP_005273709)

Publications

Species	Application	Title
mouse	WB	Cell Rep; Loss of Stathmin-2, a hallmark of TDP-43-associated ALS, causes motor neuropathy.; Authors - Kelsey L Krus
	IF	Genome Med; Spatial transcriptomic analysis of Sonic hedgehog medulloblastoma identifies that the loss of heterogeneity and promotion of differentiation underlies the response to CDK4/6 inhibition; Authors - Tuan Vo