Anticorps Polyclonal de lapin anti-STAU1

• Phare
• Validé par KD/KO

STAU1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 14225-1-AP

Synonymes

STAU, STAU1

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Galerie de données de validation

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  WB analysis of human brain using 14225-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14225-1-AP (STAU1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14225-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14225-1-AP (STAU1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14225-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14225-1-AP (STAU1 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of K-562 using 14225-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14225-1-AP (STAU1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14225-1-AP

  IP result of anti-STAU1 (IP:14225-1-AP, 4ug; Detection:14225-1-AP 1:500) with mouse brain tissue lysate 3440ug.

• 

  IHC staining of mouse brain using 14225-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14225-1-AP (STAU1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 14225-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14225-1-AP (STAU1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 14225-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14225-1-AP (STAU1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 14225-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using STAU1 antibody (14225-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules K-562, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu pancréatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• RIP: See 4 publications below

Informations sur le produit

14225-1-AP cible STAU1 dans les applications de WB, IHC, IF/ICC, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STAU1 Protéine recombinante Ag5464
• Nom complet: staufen, RNA binding protein, homolog 1 (Drosophila)
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC050432
• Symbole du gène: STAU1
• Identification du gène (NCBI): 6780
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Staufen1 (STAU1)-mediated mRNA decay (SMD) degrades translationally active mRNAs that bind the double-stranded (ds)RNA binding protein STAU1 within their 3'-untranslated regions (3'UTRs) [PMID:21307942]. STAU1 can binds to stem structures and protect Dvl2 mRNA from degradation by associating with the secondary structure in the U1 region which is located at 5′ position of second U stretch in the U2 region [PMID:17510634]. It has a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site. There are two isoforms of STAU1 protein and the molecular weight of two isoforms are repectively 63 kDa and 55 kDa

Publications

Species	Application	Title
human	WB	Nature; hiCLIP reveals the in vivo atlas of mRNA secondary structures recognized by Staufen 1.; Authors - Yoichiro Sugimoto ; KD Validated
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
	RIP	Mol Cell; Gain of Additional BIRC3 Protein Functions through 3'-UTR-Mediated Protein Complex Formation.; Authors - Shih-Han Lee
human,mouse	WB,IHC,RIP	Nat Commun; Genome-wide enhancer-gene regulatory maps link causal variants to target genes underlying human cancer risk; Authors - Pingting Ying
human	WB	Neuron; Axonal Transport of TDP-43 mRNA Granules Is Impaired by ALS-Causing Mutations.; Authors - Nael H Alami
human	WB	Nucleic Acids Res; Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.; Authors - Evan P Booy