Anticorps Monoclonal anti-STAT2

STAT2 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

1G12C4

N° de cat : 66485-1-Ig

Synonymes

ISGF 3, P113, STAT113, STAT2

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Galerie de données de validation

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  WB analysis using 66485-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66485-1-Ig (STAT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66485-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66485-1-Ig (STAT2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Ramos using 66485-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Ramos cells using STAT2 antibody (66485-1-Ig, Clone: 1G12C4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of Ramos using 66485-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Ramos cells using STAT2 antibody (66485-1-Ig, Clone: 1G12C4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules A549, cellules COLO 320, cellules PC-3
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules Ramos,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

66485-1-Ig cible STAT2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: STAT2 Protéine recombinante Ag10168
• Nom complet: signal transducer and activator of transcription 2, 113kDa
• Masse moléculaire calculée: 851 aa, 98 kDa
• Poids moléculaire observé: 113 kDa
• Numéro d’acquisition GenBank: BC051284
• Symbole du gène: STAT2
• Identification du gène (NCBI): 6773
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STAT2, also named as p113, belongs to the transcription factor STAT family. It is a signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IF stimulated genes, which drive the cell in an antiviral state. It also interacts with CRSP2, CRSP6, Simian virus 5 protein V, rabies virus phosphoprotein, IFNAR1 and IFNAR2. Its interaction with dengue virus NS5 inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), STAT2 is targeted for degradation. The calculated molecular weight of STAT2 is 98 kDa, but phosphorylated STAT2 is about 100-113 kDa.

Publications

Species	Application	Title
human	WB,IF	Int Immunopharmacol; Silencing SIRT1 promotes the anti-HBV action of IFN-α by regulating Pol expression and activating the JAK-STAT signaling pathway; Authors - Qinyan Tang
mouse	WB	Exp Eye Res; Integrated analysis of murine cornea identifies JAK/STAT signaling pathway upregulated specifically in female Vitamin A Deficient mice; Authors - Hanrui Yu
pig	WB,IP	PLoS Pathog; Free ISG15 inhibits Pseudorabies virus infection by positively regulating type I IFN signaling; Authors - Huimin Liu
mouse	WB	J Nanobiotechnology; Apoptotic tumor cell-derived microparticles loading Napabucasin inhibit CSCs and synergistic immune therapy; Authors - Boping Jing