Anticorps Polyclonal de lapin anti-STAR

STAR Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris et plus (2)

Applications

WB, IHC, IF-Fro, ELISA

Conjugaison

Non conjugué

Publications

42

N° de cat : 12225-1-AP

Synonymes

STAR, STARD1, steroidogenic acute regulatory protein

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Galerie de données de validation

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  WB analysis using 12225-1-AP

  Pig adrenal gland lysates were subjected to SDS PAGE followed by western blot with 12225-1-AP (STAR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human adrenal gland using 12225-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 12225-1-AP (STAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 12225-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12225-1-AP (STAR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12225-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12225-1-AP (STAR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 12225-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12225-1-AP (STAR Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse testis using 12225-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12225-1-AP (STAR Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary using 12225-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 12225-1-AP (STAR Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 12225-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 12225-1-AP (STAR Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse testis using 12225-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using STAR antibody (12225-1-AP) at dilution of 1:200 and CoraLite®647-conjugated AffiniPure F(ab, CoraLite® Plus 488 BOULE antibody (CL488-13720, green), CoraLite®555 TNP1 antibody (CL555-17178, orange).

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Applications testées

• Résultats positifs en WB: tissu de glande surrénale de porc, tissu de glande surrénale humain
• Résultats positifs en IHC: tissu testiculaire de souris, tissu de cancer du foie humain, tissu ovarien humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-Fro: tissu testiculaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 32 publications below
• IHC: See 8 publications below
• IF: See 6 publications below

Informations sur le produit

12225-1-AP cible STAR dans les applications de WB, IHC, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Chèvre, Humain, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STAR Protéine recombinante Ag2862
• Nom complet: steroidogenic acute regulatory protein
• Masse moléculaire calculée: 285 aa, 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC010550
• Symbole du gène: STAR
• Identification du gène (NCBI): 6770
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Steroidogenic acute regulatory protein (StAR) is a key player in acute regulation of hormone-induced steroidogenesis, by facilitating cholesterol transport from cellular stores to inner mitochondria membrane where the cholesterol was conversed to pregnenolone. StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue.

Publications

Species	Application	Title
human	WB	Transl Res; Induction of autophagy by Beclin-1 in granulosa cells contributes to follicular progesterone elevation in ovarian endometriosis.; Authors - Ying Ding
human	IHC	Clin Transl Med; Single-cell transcriptomes reveal heterogeneity of high-grade serous ovarian carcinoma.; Authors - Qian Hao
mouse	WB	Int J Nanomedicine; La2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice.; Authors - Lu Yuan
mouse	WB	Ecotoxicol Environ Saf; FDX1 regulates leydig cell ferroptosis mediates PM2.5-induced testicular dysfunction of mice; Authors - Lipeng Li
mouse	WB	Environ Pollut; Prenatal exposure to propylparaben at human-relevant doses accelerates ovarian aging in adult mice.; Authors - Milu Li
mouse	WB	Sci Rep; STMN1 Promotes Progesterone Production Via StAR Up-regulation in Mouse Granulosa Cells.; Authors - Yun-De Dou