Anticorps Polyclonal de lapin anti-STAM

• Phare
• Validé par KD/KO

STAM Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

18

N° de cat : 12434-1-AP

Synonymes

STAM, STAM 1, STAM1

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Galerie de données de validation

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  WB analysis using 12434-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12434-1-AP (STAM antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Multi-cells using 12434-1-AP

  WB result of 12434-1-aP (STAM antibody).

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  WB analysis of mouse brain using 12434-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12434-1-AP (STAM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 12434-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12434-1-AP (STAM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 12434-1-AP

  IP result of anti-STAM (IP:12434-1-AP, 4ug; Detection:12434-1-AP 1:300) with PC-3 cells lysate 1440ug.

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  IHC staining of human testis using 12434-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 12434-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse testis using 12434-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 12434-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12434-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using STAM antibody (12434-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12434-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 12434-1-AP (STAM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules C2C12, cellules Jurkat, cellules K-562, cellules MCF-7, cellules PC-3, cellules Raji, cellules SKOV-3, cellules Y79, multi-cellules, tissu cérébral de souris
• Résultats positifs en IP: cellules PC-3
• Résultats positifs en IHC: tissu testiculaire humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 15 publications below
• IF: See 8 publications below

Informations sur le produit

12434-1-AP cible STAM dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STAM Protéine recombinante Ag3112
• Nom complet: signal transducing adaptor molecule (SH3 domain and ITAM motif) 1
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC030586
• Symbole du gène: STAM
• Identification du gène (NCBI): 8027
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STAM, also named as STAM1, belongs to the STAM family. It is involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. STAM may also play a role in T-cell development. It is involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs). Together with HRS, STAM forms ESCRT-0. STAM was originally identified as an adaptor protein involved in cytokine signaling.(PMID:20505072)

Publications

Species	Application	Title
human	WB	Nature; A phosphoinositide signalling pathway mediates rapid lysosomal repair; Authors - Jay Xiaojun Tan
human	IF	Nat Cell Biol; Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.; Authors - Vincent Mercier
human	WB	Mol Cell; Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.; Authors - Susanne Zellner
human	WB,IF	Cell Rep; TIM-1 Ubiquitination Mediates Dengue Virus Entry.; Authors - Ophélie Dejarnac ; KD Validated
		Development; Identification of STAM1 as a novel effector of ventral projection of spinal motor neurons.; Authors - Heejin Nam
human	IF,WB	PLoS Pathog; A combined EM and proteomic analysis places HIV-1 Vpu at the crossroads of retromer and ESCRT complexes: PTPN23 is a Vpu-cofactor.; Authors - Charlotte A Stoneham