Anticorps Polyclonal de lapin anti-ST8SIA1

• Phare
• Validé par KD/KO

ST8SIA1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA, IF

Conjugaison

Non conjugué

Publications

7

N° de cat : 24918-1-AP

Synonymes

Alpha 2,8 sialyltransferase 8A, Ganglioside GD3 synthase, Ganglioside GT3 synthase, GD3S, Sialyltransferase 8A, Sialytransferase St8Sia I, SIAT8, SIAT8 A, SIAT8A, ST8SIA1, ST8SiaI

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Galerie de données de validation

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  WB analysis using 24918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24918-1-AP (ST8SIA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 24918-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24918-1-AP (ST8SIA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 24918-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 24918-1-AP (ST8SIA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 24918-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 24918-1-AP (ST8SIA1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 24918-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 24918-1-AP (ST8SIA1 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A375, cellules MCF-7, cellules MDA-MB-453s, cellules SH-SY5Y
• Résultats positifs en IHC: tissu cutané humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

24918-1-AP cible ST8SIA1 dans les applications de WB, IHC, ELISA, IF et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ST8SIA1 Protéine recombinante Ag20429
• Nom complet: ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1
• Masse moléculaire calculée: 356 aa, 41 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC126162
• Symbole du gène: ST8SIA1
• Identification du gène (NCBI): 6489
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ST8SIA1 is a member of the ST family involved in the production of gangliosides. ST8SIA1 is strongly expressed in melanoma cell lines, adult and fetal brain, and to a lesser extent in adult and fetal lung. The abnormal expression of ST8SIA1 has been demonstrated to influence the metastasis of triple-negative breast cancer. (PMID: 34429775)

Publications

Species	Application	Title
	IF	Mol Oncol; Upregulation of cell surface GD3 ganglioside phenotype is associated with human melanoma brain metastasis.; Authors - Romela Irene Ramos
human	IHC	Front Pharmacol; Identifying an Immune-Related Gene ST8SIA1 as a Novel Target in Patients With Clear-Cell Renal Cell Carcinoma.; Authors - Xu Hu
human	IHC	Oncol Lett; ST8SIA1 inhibits the proliferation, migration and invasion of bladder cancer cells by blocking the JAK/STAT signaling pathway.; Authors - Shengjin Yu
mouse	WB	bioRxiv; Role of GD2 and its biosynthetic enzyme GD3 synthase in prostate cancer tumorigenesis; Authors - Aaqib M Bhat ; KO Validated
mouse	WB	Sci Rep; GD2 and its biosynthetic enzyme GD3 synthase promote tumorigenesis in prostate cancer by regulating cancer stem cell behavior; Authors - Aaqib M Bhat
human	IHC	Front Physiol; Atlas of human dental pulp cells at multiple spatial and temporal levels based on single-cell sequencing analysis; Authors - Huihui Ren