Anticorps Polyclonal de lapin anti-ST7L

ST7L Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 17567-1-AP

Synonymes

FAM4B, ST7 related protein, ST7L, ST7R, STLR

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Galerie de données de validation

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  WB analysis using 17567-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17567-1-AP (ST7L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17567-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17567-1-AP (ST7L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 17567-1-AP

  IP result of anti-ST7L (IP:17567-1-AP, 3ug; Detection:17567-1-AP 1:1500) with mouse testis tissue lysate 5000ug.

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Applications testées

• Résultats positifs en WB: cellules L02, cellules MCF-7, cellules T-47D
• Résultats positifs en IP: tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

17567-1-AP cible ST7L dans les applications de WB, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ST7L Protéine recombinante Ag11768
• Nom complet: suppression of tumorigenicity 7 like
• Masse moléculaire calculée: 575 aa, 65 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC065501
• Symbole du gène: ST7L
• Identification du gène (NCBI): 54879
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ST7 (suppressor of tumorigenicity 7) gene has been proposed to be a tumor suppressor gene, product of which is a multi-pass membrane protein belonging to the ST7 family. ST7L (suppressor of tumorigenicity 7 protein-like) gene encodes a multi-pass membrane protein also belonging to the ST7 family, 8 isoforms produced by alternative splicing have been described . However, the molecular function of this gene product has not been determined so far.

Publications

Species	Application	Title
human	WB,CoIP	Cell Death Dis; MicroRNA-23b functions as an oncogene and activates AKT/GSK3β/β-catenin signaling by targeting ST7L in hepatocellular carcinoma.; Authors - Likun Zhuang
human	WB	J Exp Clin Cancer Res; Long noncoding RNA MIR31HG inhibits hepatocellular carcinoma proliferation and metastasis by sponging microRNA-575 to modulate ST7L expression.; Authors - Shaoying Yan
human	WB	Int J Mol Med; miR-378 functions as an onco-miRNA by targeting the ST7L/Wnt/β-catenin pathway in cervical cancer.; Authors - Shengjie Li