Anticorps Polyclonal de lapin anti-ST3GAL5

• Phare
• Validé par KD/KO

ST3GAL5 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 14614-1-AP

Synonymes

ST3GAL5, ST3GalV, UNQ2510/PRO5998, CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransferase, EC:2.4.3.9, Lactosylceramide alpha-2,3-sialyltransferase, SIAT9, SIATGM3S, ST3Gal V

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Galerie de données de validation

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  WB analysis of HEK-293 using 14614-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14614-1-AP (ST3GAL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 14614-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14614-1-AP (ST3GAL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 14614-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14614-1-AP (ST3GAL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14614-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14614-1-AP (ST3GAL5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14614-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14614-1-AP (ST3GAL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 14614-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14614-1-AP (ST3GAL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 14614-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14614-1-AP (ST3GAL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 14614-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14614-1-AP (ST3GAL5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 14614-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14614-1-AP (ST3GAL5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 14614-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14614-1-AP (ST3GAL5 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 14614-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14614-1-AP (ST3GAL5 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, tissu placentaire humain, tissu testiculaire humain
• Résultats positifs en IHC: tissu de muscle squelettique de souris, tissu cutané humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

14614-1-AP cible ST3GAL5 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ST3GAL5 Protéine recombinante Ag6207
• Nom complet: ST3 beta-galactoside alpha-2,3-sialyltransferase 5
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC065936
• Symbole du gène: ST3GAL5
• Identification du gène (NCBI): 8869
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ST3GAL5 is also named as SIAT9, GM3 synthase, ST3Gal V, Sialyltransferase 9 and belongs to the glycosyltransferase 29 family. ST3GAL5 transfers the sialyl group (N-acetyl-alpha-neuraminyl or NeuAc) from CMP-NeuAc to the non-reducing terminal galactose of glycosphingolipids forming gangliosides (PMID:9822625, PMID:16934889). SIAT9 is mainly involved in the biosynthesis of ganglioside GM3 but can also use different glycolipids as substrate acceptors such as D-galactosylceramide (GalCer), asialo-GM2 (GA2) and asialo-GM1 (GA1) (PMID:16934889). ST3GAL5 is highly expressed in brain, skeletal muscle, placenta, and testis. And mRNA of ST3GAL5 is widely distributed in human brain, but slightly elevated expression was observed in the cerebral cortex, temporal lobe, and putamen (PMID:9822625). ST3GAL5 has 3 isoforms with the molecular mass of 45, 46, and 48 kDa. Sometimes the band of 70 kDa can also be observed, which may be a glycosylated form of ST3GAL5 (PMID: 34943806, PMID: 26458842).

Publications

Species	Application	Title
human	IHC	Front Immunol; Identification of ST3GAL5 as a prognostic biomarker correlating with CD8+ T cell exhaustion in clear cell renal cell carcinoma; Authors - Jiakuan Liu
human	WB	Front Cell Dev Biol; PRC2-dependent regulation of ganglioside expression during dedifferentiation contributes to the proliferation and migration of vascular smooth muscle cells; Authors - Norihiko Sasaki
human	WB	iScience; C-Myc-induced hypersialylation of small cell lung cancer facilitates pro-tumoral phenotypes of macrophages; Authors - Lin Tian
human,mouse	WB,IHC,IF	Mol Oncol; Exosomes secreted by ST3GAL5high cancer cells promote peritoneal dissemination by establishing a pre-metastatic microenvironment; Authors - Misato Horie ; KO Validated