Anticorps Polyclonal de lapin anti-ST3GAL2

ST3GAL2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 13620-1-AP

Synonymes

Alpha 2,3 ST 2, Gal NAc6S, Sialyltransferase 4B, SIAT4 B, SIAT4B, ST3Gal II, ST3GAL2, ST3GalA.2, ST3GALII

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Galerie de données de validation

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  WB analysis of human colon using 13620-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13620-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13620-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 13620-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 13620-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13620-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat heart using 13620-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 13620-1-AP (ST3GAL2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 13620-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 13620-1-AP (ST3GAL2 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 13620-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 13620-1-AP (ST3GAL2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 13620-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 13620-1-AP (ST3GAL2 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu de côlon humain, cellules HL-60, cellules K-562, tissu cardiaque humain, tissu hépatique humain, tissu splénique humain
• Résultats positifs en IHC: tissu cardiaque de rat, tissu cutané humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

13620-1-AP cible ST3GAL2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ST3GAL2 Protéine recombinante Ag4528
• Nom complet: ST3 beta-galactoside alpha-2,3-sialyltransferase 2
• Masse moléculaire calculée: 350 aa, 40 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC036777
• Symbole du gène: ST3GAL2
• Identification du gène (NCBI): 6483
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Beta-galactoside alpha-2,3-sialyltransferase 2 (ST3GAL2) is a sialyltransferase (ST) that transfers sialic acid from the activated donor CMP-sialic acid to the glycan terminus of a glycolipid or glycoprotein. ST3GAL2 contains two putative N-glycosylation sites (Asn92 and Asn211), which are mainly responsible for GD1a and GT1b ganglioside biosynthesis in the brain. ST3GAL2 belongs to the sialyltransferase family, which mediates the terminal modification of glycoproteins and glycolipids. ST3GAL2 has been found to play a role in obesity, aging, and malignant diseases (PMID: 34319453). The calculated molecular weight of ST3GAL2 is 40 kDa, but the observed is 55 kDa, which may be the glycosylation form of ST3GAL2.