Anticorps Monoclonal anti-SSTR5

SSTR5 Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

2B10D8

N° de cat : 66772-1-Ig

Synonymes

somatostatin receptor 5, Somatostatin receptor type 5, SS 5 R, SS5 R, SS5R, SST5, SSTR5

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Galerie de données de validation

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  WB analysis using 66772-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66772-1-Ig (SSTR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66772-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66772-1-Ig (SSTR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse kidney using 66772-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using SSTR5 antibody (66772-1-Ig, Clone: 2B10D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse kidney using 66772-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using SSTR5 antibody (66772-1-Ig, Clone: 2B10D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HSC-T6, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Neuro-2a, cellules NIH/3T3
• Résultats positifs en IF: tissu rénal de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

66772-1-Ig cible SSTR5 dans les applications de WB, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SSTR5 Protéine recombinante Ag18615
• Nom complet: somatostatin receptor 5
• Masse moléculaire calculée: 364 aa, 39 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC146576
• Symbole du gène: SSTR5
• Identification du gène (NCBI): 6755
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Somatostatin is a widely distributed peptide with a broad range of biological actions. Two biological forms of somatostatin exist: somatostatin-14 and -28. Somatostatin receptors (SSTR1, 2A and B, 3, 4 and 5) belong to the G protein coupled receptor family and have a wide expression pattern in both normal tissues and solid tumors (PMID: 23872332). SSTR5 has greater affinity for somatostatin-28 than somatostatin-14 (PMID: 8373420).

Publications

Species	Application	Title
rat	WB	Phytomedicine; Differential effect of polysaccharide and nonpolysaccharide components in Sijunzi decoction on spleen deficiency syndrome and their mechanisms.; Authors - Ping Ma
mouse	WB	Endocr J; Differential effects of β-arrestin1 and β-arrestin2 on somatostatin receptors in murine AtT-20 corticotroph tumor cells.; Authors - Kazunori Kageyama
human	IF	Front Endocrinol (Lausanne); Dimerization of GPCRs: Novel insight into the role of FLNA and SSAs regulating SST2 and SST5 homo- and hetero-dimer formation; Authors - Donatella Treppiedi
human	WB,CoIP	Cell Death Discov; Repositioning Lomitapide to block ZDHHC5-dependant palmitoylation on SSTR5 leads to anti-proliferation effect in preclinical pancreatic cancer models; Authors - Yumeng Wang