Anticorps Polyclonal de lapin anti-SRY

SRY Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 17930-1-AP

Synonymes

sex determining region Y, SRY, TDF, TDY, Testis determining factor

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Galerie de données de validation

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  WB analysis of human testis using 17930-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 17930-1-AP (SRY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17930-1-AP (SRY antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17930-1-AP (SRY antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17930-1-AP (SRY antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17930-1-AP (SRY antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17930-1-AP (SRY antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17930-1-AP (SRY antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17930-1-AP (SRY antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17930-1-AP (SRY antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17930-1-AP (SRY antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17930-1-AP (SRY antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17930-1-AP (SRY antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17930-1-AP (SRY antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain
• Résultats positifs en IHC: tissu testiculaire de souris, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

17930-1-AP cible SRY dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SRY Protéine recombinante Ag12360
• Nom complet: sex determining region Y
• Masse moléculaire calculée: 204 aa, 24 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC074924
• Symbole du gène: SRY
• Identification du gène (NCBI): 6736
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SRY (sex-determining region Y protein) is a tran-scriptional activator required for male sex determination in mammals. This protein, also referred to as testis-determining factor (TDF), is an HMG box protein that initiates the formation of testis from undifferentiated gonad. The DNA-binding activity of SRY is required for normal testis formation. This DNA-binding activity is thought to be regulated by PKA, which phosphorylates SRY in vivo. Mutations in SRY have been associated with 46,XY gonadal dysgenesis, in which the gonads fail to develop in XY phenotypic females.