Anticorps Polyclonal de lapin anti-SRX1

• Phare
• Validé par KD/KO

SRX1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

32

Avis (2)

N° de cat : 14273-1-AP

Synonymes

C20orf139, SRX, SRX1, SRXN1, Sulfiredoxin 1, YKL086W

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Galerie de données de validation

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  WB analysis using 14273-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14273-1-AP (SRX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14273-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14273-1-AP (SRX1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14273-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14273-1-AP (SRX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 14273-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 14273-1-AP (SRX1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 14273-1-AP

  IP result of anti-SRX1 (IP:14273-1-AP, 3ug; Detection:14273-1-AP 1:400) with A549 cells lysate 3300ug.

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  IHC staining of human lung cancer using 14273-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14273-1-AP (SRX1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 14273-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14273-1-AP (SRX1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 14273-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14273-1-AP (SRX1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14273-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14273-1-AP (SRX1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 14273-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14273-1-AP (SRX1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14273-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14273-1-AP (SRX1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293 transfectées, cellules HepG2, cellules U-251, tissu hépatique de souris
• Résultats positifs en IP: cellules A549,
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du sein humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 25 publications below
• IHC: See 13 publications below
• IF: See 2 publications below

Informations sur le produit

14273-1-AP cible SRX1 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SRX1 Protéine recombinante Ag5613
• Nom complet: sulfiredoxin 1 homolog (S. cerevisiae)
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC047707
• Symbole du gène: SRXN1
• Identification du gène (NCBI): 140809
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse		Nat Commun; Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle.; Authors - John T Olthoff
human	WB,IHC	Proc Natl Acad Sci U S A; Sulfiredoxin-Peroxiredoxin IV axis promotes human lung cancer progression through modulation of specific phosphokinase signaling.; Authors - Wei Qiou Q
human	IHC	Cancer Res; Genetic polymorphisms and protein expression of NRF2 and Sulfiredoxin predict survival outcomes in breast cancer.; Authors - Hartikainen Jaana M JM
human	WB	Oncogene; PMID: 28504720; Authors - E-J Choi
human,mouse	WB,IHC	Cancer Lett; Nrf2-activated expression of sulfiredoxin contributes to urethane-induced lung tumorigenesis.; Authors - Murli Mishra ; KD Validated
mouse	WB,IHC	Carcinogenesis; Tumor Promoter-induced Sulfiredoxin Is Required for Mouse Skin Tumorigenesis.; Authors - Lisha Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shinford (Verified Customer) (09-23-2023)

Detecting lower molecular weight molecules in gels is relatively difficult, we tried several antibodies from different companies and this worked well at a concentration of 1:500.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Breast cancer cells

SRX1 Antibody Western Blot validation (1:500 dilution) in Breast cancer cells (Cat no:14273-1-AP)

FH

Christopher (Verified Customer) (12-03-2018)

Primary neurons were lysed in RIPA and 20ug samples (containing 1x Laemmli) were run on Bis-Tris gels in 1xMOPS buffer. This antibody recognised SRXN-1 in HEK293FT cells transfected with Human SRXN1 at 17kDa. In HEK cells, non specific bands were visible at around 28 and 35kDa as well.In primary neurons, the antibody recognised a band at roughly 15kDa however it binds non sepcifically to the Pageruler Prestained protein ladder which is an issue.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Primary cortical neurons and transfected HEK293FT cells