Anticorps Polyclonal de lapin anti-SRP68

SRP68 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 11585-1-AP

Synonymes

SRP68

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Galerie de données de validation

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  WB analysis of mouse brain using 11585-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11585-1-AP (SRP68 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11585-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11585-1-AP (SRP68 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 11585-1-AP

  IP result of anti-SRP68 (IP:11585-1-AP, 4ug; Detection:11585-1-AP 1:400) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human gliomas using 11585-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11585-1-AP (SRP68 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 11585-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11585-1-AP (SRP68 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 11585-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11585-1-AP (SRP68 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11585-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11585-1-AP (SRP68 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11585-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SRP68 antibody (11585-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu de gliome humain, tissu testiculaire de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below

Informations sur le produit

11585-1-AP cible SRP68 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SRP68 Protéine recombinante Ag2177
• Nom complet: signal recognition particle 68kDa
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC020238
• Symbole du gène: SRP68
• Identification du gène (NCBI): 6730
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of secretory proteins to the rough endoplasmic reticulum (ER) membrane. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function. SRP68 is one component of the ribonucleprotein complex, and forms a heterodimer with SRP72.

Publications

Species	Application	Title
mouse	WB	Nat Commun; The Perlman syndrome DIS3L2 exoribonuclease safeguards endoplasmic reticulum-targeted mRNA translation and calcium ion homeostasis.; Authors - Mehdi Pirouz
	WB	Nucleic Acids Res; The pioneer round of translation ensures proper targeting of ER and mitochondrial proteins.; Authors - Joori Park
human	WB	Nucleic Acids Res; Implication of the SMN complex in the biogenesis and steady state level of the signal recognition particle.; Authors - Piazzon Nathalie N
human	WB	Nucleic Acids Res; Protein Hit1, a novel box C/D snoRNP assembly factor, controls cellular concentration of the scaffolding protein Rsa1 by direct interaction.; Authors - Benjamin Rothé
human	WB	PLoS One; Decreased dicer expression enhances SRP-mediated protein targeting.; Authors - Ren Yong-Feng YF
human	WB	PLoS One; The CELF1 RNA-Binding Protein Regulates Decay of Signal Recognition Particle mRNAs and Limits Secretion in Mouse Myoblasts.; Authors - Joseph Russo