Anticorps Polyclonal de lapin anti-SRP54

SRP54 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 11729-1-AP

Synonymes

SRP54

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Galerie de données de validation

• 

  WB analysis of human placenta using 11729-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11729-1-AP (SRP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 11729-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11729-1-AP (SRP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 11729-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11729-1-AP (SRP54 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 11729-1-AP

  IP result of anti-SRP54 (IP:11729-1-AP, 4ug; Detection:11729-1-AP 1:300) with human placenta tissue lysate 1015 ug.

• 

  IHC staining of human breast cancer using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, cellules NIH/3T3
• Résultats positifs en IP: tissu placentaire humain,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

11729-1-AP cible SRP54 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SRP54 Protéine recombinante Ag2327
• Nom complet: signal recognition particle 54kDa
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC003389
• Symbole du gène: SRP54
• Identification du gène (NCBI): 6729
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).

Publications

Species	Application	Title
human		Mol Cell; Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover.; Authors - Dianrong Li
human	WB	PLoS One; Decreased dicer expression enhances SRP-mediated protein targeting.; Authors - Ren Yong-Feng YF
human	WB	Biochem Biophys Res Commun; Polyglutamine expansion disturbs the endoplasmic reticulum formation, leading to caspase-7 activation through Bax.; Authors - Masashi Ueda
human	WB	Biochem Biophys Res Commun; Expanded polyglutamine embedded in the endoplasmic reticulum causes membrane distortion and coincides with Bax insertion.; Authors - Masashi Ueda
human	WB	iScience; Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges; Authors - Tamami Miyagi