Anticorps Polyclonal de lapin anti-SRP19

SRP19 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 16033-1-AP

Synonymes

SRP19

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Galerie de données de validation

• 

  WB analysis of human liver using 16033-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16033-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16033-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16033-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 16033-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16033-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 16033-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16033-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 16033-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 16033-1-AP

  IP result of anti-SRP19 (IP:16033-1-AP, 4ug; Detection:16033-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

• 

  IF Staining of HeLa using 16033-1-AP

  Immunofluorescent analysis of HeLa cells using 16033-1-AP (SRP19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu hépatique humain, cellules A549, cellules HeLa, cellules K-562, cellules Raji, tissu hépatique de souris, tissu ovarien de souris, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

16033-1-AP cible SRP19 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SRP19 Protéine recombinante Ag8903
• Nom complet: signal recognition particle 19kDa
• Masse moléculaire calculée: 144 aa, 16 kDa
• Poids moléculaire observé: 18-25 kDa
• Numéro d’acquisition GenBank: BC010947
• Symbole du gène: SRP19
• Identification du gène (NCBI): 6728
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The signal recognition particle (SRP) is one of the few functional small RNP particles. The SRP couples the synthesis of membrane and secretory proteins across or into the endoplasmic reticulum (ER) membrane in eukaryotes, as well as across the bacterial plasma membrane, and chloroplast thylakoid membranes. The mammalian SRP is composed of a 7S (or 7SL) RNA and six different proteins, SRP9, SRP14, SRP19, SRP54, SRP68 and SRP72. All of the components of SRP, including SRP RNA, participate directly in the overall protein targeting process. SRP19 binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP. SRP19 was shown to significantly enhance SRP54 attachment to helix 8 of 7SL RNA. Binding of SRP19 leads to restructuring of both helix 6 and 8, causing local changes at the SRP54-binding site. This antibody is a rabbit polyclonal antibody raised against full length SRP19 of human origin.

Publications

Species	Application	Title
	WB	Nucleic Acids Res; The pioneer round of translation ensures proper targeting of ER and mitochondrial proteins.; Authors - Joori Park
human	WB	Nucleic Acids Res; SECIS-binding protein 2 interacts with the SMN complex and the methylosome for selenoprotein mRNP assembly and translation.; Authors - Anne-Sophie Gribling-Burrer
human	WB	PLoS One; The CELF1 RNA-Binding Protein Regulates Decay of Signal Recognition Particle mRNAs and Limits Secretion in Mouse Myoblasts.; Authors - Joseph Russo
human	WB	Elife; The unfolded protein response and endoplasmic reticulum protein targeting machineries converge on the stress sensor IRE1.; Authors - Diego Acosta-Alvear