Anticorps Polyclonal de lapin anti-SRGAP2

• Phare
• Validé par KD/KO

SRGAP2 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, ELISA, IF

Conjugaison

Non conjugué

Publications

4

N° de cat : 22519-1-AP

Synonymes

SRGAP2A, SLIT-ROBO Rho GTPase-activating protein 2, Rho GTPase-activating protein 34, Formin-binding protein 2, FNBP2

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Galerie de données de validation

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  WB analysis of mouse kidney using 22519-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22519-1-AP (SRGAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 22519-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22519-1-AP (SRGAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22519-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22519-1-AP (SRGAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 22519-1-AP

  IP result of anti-SRGAP2 (IP:22519-1-AP, 4ug; Detection:22519-1-AP 1:1000) with HEK-293 cells lysate 3000ug.

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  IHC staining of human kidney using 22519-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 22519-1-AP (SRGAP2 Antibody) at dilution of 1:50.

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  IHC staining of mouse brain using 22519-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22519-1-AP (SRGAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HEK-293
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu cérébral de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

22519-1-AP cible SRGAP2 dans les applications de WB, IHC, IP, ELISA, IF et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SRGAP2 Protéine recombinante Ag18278
• Nom complet: SLIT-ROBO Rho GTPase activating protein 2
• Masse moléculaire calculée: 1071 aa, 121 kDa
• Poids moléculaire observé: 121 kDa
• Numéro d’acquisition GenBank: BC132874
• Symbole du gène: SRGAP2
• Identification du gène (NCBI): 23380
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLIT-ROBO Rho GTPase-activating protein 2 (SRGAP2) is also named as ARHGAP34, FNBP2, KIAA0456 and SRGAP2A. SRGAP2 plays important roles in axon guidance, neuronal migration, synapse formation, and nerve regeneration. SRGAP2 protein was first expressed in the retina of normal mice at the embryonic stage and was mainly located in the mature retinal ganglion cell layer and the inner nuclear layer (PMID: 37056152). SRGAP2 plays a key role in neuronal morphogenesis and migration mainly during development of the cerebral cortex (PMID: 20810653, PMID: 27373832, PMID: 28333212). SRGAP2 are critical for neuronal migration through inactivation of Rho GTPases Cdc42, Rac1, and RhoA (PMID: 20810653). SRGAP2A promotes synaptic maturation, and ultimately the synaptic accumulation of AMPA and GABAA receptors, by interacting with key components of both excitatory and inhibitory postsynaptic scaffolds, Homer and Gephyrin (PMID: 27373832).

Publications

Species	Application	Title
human	WB,IHC,IF	Theranostics; Stiff matrix induced srGAP2 tension gradients control migration direction in triple-negative breast cancer; Authors - Chen Li ; KD Validated
human	WB	Nat Commun; Haploinsufficiency in the ANKS1B gene encoding AIDA-1 leads to a neurodevelopmental syndrome.; Authors - Abigail U Carbonell
mouse	WB	Cell Rep; Huntingtin coordinates dendritic spine morphology and function through cofilin-mediated control of the actin cytoskeleton; Authors - Doris Wennagel
mouse	WB,IHC	Biomed Pharmacother; CDDO-Me ameliorates podocyte injury through anti-oxidative stress and regulation of actin cytoskeleton in adriamycin nephropathy; Authors - Cheng Xu