Anticorps Polyclonal de lapin anti-SPRED2

• Phare
• Validé par KD/KO

SPRED2 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 24091-1-AP

Synonymes

Spred 2, SPRED2

---

Galerie de données de validation

• 

  WB analysis of MCF-7 using 24091-1-AP

  WB result of SPRED2 antibody (24091-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPRED2 transfected MCF-7 cells.

• 

  WB analysis using 24091-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24091-1-AP (SPRED2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24091-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24091-1-AP (SPRED2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 24091-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24091-1-AP (SPRED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 24091-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24091-1-AP (SPRED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 24091-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 24091-1-AP (SPRED2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 24091-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 24091-1-AP (SPRED2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 24091-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24091-1-AP (SPRED2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 24091-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24091-1-AP (SPRED2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat testis using 24091-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 24091-1-AP (SPRED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 24091-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 24091-1-AP (SPRED2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 24091-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 24091-1-AP (SPRED2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules PC-12, cellules MCF-7, cellules T-47D, tissu cérébral de rat, tissu cérébral de souris, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu testiculaire de rat, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

24091-1-AP cible SPRED2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPRED2 Protéine recombinante Ag21359
• Nom complet: sprouty-related, EVH1 domain containing 2
• Masse moléculaire calculée: 418 aa, 48 kDa
• Poids moléculaire observé: 40-48 kDa
• Numéro d’acquisition GenBank: BC136334
• Symbole du gène: SPRED2
• Identification du gène (NCBI): 200734
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPRED2 is a member of the SPRED family of proteins that modulate GFR signaling by inhibiting the Ras-MAPK cascade (PMID: 17094949). SPRED family members are characterized by an N-terminal Enabled/VASP homology 1 domain (EVH1), a central c-Kit binding domain (KBD) and a C-terminal Sprouty-related domain (SPR) (PMID: 17691106). Three mammalian SPREDs (SPRED1-3) have been described. In adult tissues, SPRED2 is expressed ubiquitously, but most highly expressed in glandular epithelia (PMID: 15580519; 17691106). Reduced expression level of SPRED2 has been reported in human hepatocellular carcinoma (HCC), suggesting SPRED2 may play a role in tumorgenesis (PMID: 16652141). Gene disruption of SPRED2 causes dwarfism (PMID: 15946934).

Publications

Species	Application	Title
human	IHC	PLoS One; Expression of Spred2 in the urothelial tumorigenesis of the urinary bladder; Authors - Shinsuke Oda
mouse	WB	Biol Pharm Bull; Inhibition of Spred/Sprouty Expression in the Skin of a Contact Dermatitis-Like Model.; Authors - Hiroyasu Sakai
mouse	IF	Histol Histopathol; microRNA-141-3p mediates epithelial cell proliferation, apoptosis, and epithelial-mesenchymal transition and alleviates pulmonary fibrosis in mice via Spred2; Authors - Liang Zhu
human	WB	Int J Mol Sci; SPRED2: A Novel Regulator of Epithelial-Mesenchymal Transition and Stemness in Hepatocellular Carcinoma Cells; Authors - Tong Gao